Abstract
The taxonomic status of a moderately halophilic bacterium, strain N4T, isolated from soil of a chicken farm in China was determined. It was Gram-negative, non-spore-forming, motile, and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequence indicated that this strain belonged to the genus Salinicola, as it showed the highest sequence similarities to Salinicola salaries M27T (98.3 %), Salinicola socius SMB35T (98.1 %), and Salinicola halophilus CG4.1T (98.1 %). The major cellular fatty acids were C16:0 (25.6 %), C18:1ω7c (35.0 %), and C19:0 cyclo ω8c (11.9 %), which are properties shared by members of the genus Salinicola. The DNA G+C content of strain N4T was 69.1 mol %. The level of DNA–DNA relatedness between strain N4T and the other three type strains of the genus of Salinicola salaries M27T, Salinicola socius SMB35T, and Salinicola halophilus CG4.1T were 34.3, 28.7, and 26.9 %, respectively. Based on the results of phenotypic, chemotaxonomic, DNA–DNA relatedness, and phylogenetic analysis, strain N4T should be classified as a novel species of the genus Salinicola, for which the name Salinicola zeshunii sp. nov. is proposed, with strain N4T (=KACC 16602T = CCTCC AB 2012912T) as the type strain.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Explore related subjects
Discover the latest articles and news from researchers in related subjects, suggested using machine learning.References
Aguilera M, Cabrera A, Incerti C et al (2007) Chromohalobacter salarius sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almerı′a, southern Spain. Int J Syst Evol Microbiol 57:1238–1242
Anan’ina LN, Plotnikova EG, Gavrish E et al (2007) Salinicola socius gen. nov., sp. nov., a moderately halophilic bacterium from a naphthalene-utilizing microbial association. Mikrobiologiia 76:369–376
Anan’ina LN, Plotnikova EG, Gavrish E et al (2008) Salinicola socius sp. nov. in list of new names and new combinations previously effectively. Int J Syst Evol Microbiol 58:2471–2472
de la Haba RR, Sa’nchez-Porro C, Ma′rquez MC et al (2010) Taxonomic study of the genus Salinicola: transfer of Halomonas salaria and Chromohalobacter salarius to the genus Salinicola as Salinicola salarius comb. nov. and Salinicola halophilus nom. nov., respectively. Int J Syst Evol Microbiol 60:963–971
Ezaki T, Hashimoto Y, Yabuuchi E (1989) Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
Kim KK, Jin L, Yang HC et al (2007) Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. Int J Syst Evol Microbiol 57:675–681
Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, p 371–375
Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218
Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory 186 manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 607–654
Tindall BJ (1990) A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130
Tindall BJ (1990) Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202
Vreeland RH, Litchfield CD, Martin EL et al (1980) Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Bacteriol 30:485–495
Wayne LG, Brenner DJ, Colwell RR, International Committee on Systematic Bacteriology et al (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
Acknowledgments
This study was supported by the Chinese National Natural Science Fund (31070099, 30830001 and 31000060), Natural Science Foundation of Jiangsu Province, China (BK2009312), the Opening Fund of State Key Laboratory of Soil and Sustainable Agriculture (Y052010025), and the Major Technology Special Social Development Project (2007C13059).
Author information
Authors and Affiliations
Corresponding author
Additional information
The 16S rRNA gene sequence of strain N4T has been deposited in the GenBank/EMBL/DDBJ under the accession number EU056581.
Rights and permissions
About this article
Cite this article
Cao, L., Yan, Q., Ni, H. et al. Salinicola zeshunii sp. nov., a Moderately Halophilic Bacterium Isolated from Soil of a Chicken Farm. Curr Microbiol 66, 192–196 (2013). https://doi.org/10.1007/s00284-012-0259-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-012-0259-4