Abstract
Aeromonas P2973 was isolated from the water of a brook in a cavern in the Czech Republic. This isolate could not be biochemically identified at the species level, considering all updated species descriptions. Subsequent extensive phenotypic characterisation, DNA–DNA hybridisation, 16S rRNA gene sequencing and a Multi-Locus Phylogenetic Analysis (MLPA) of the concatenated sequence of 7 housekeeping genes (gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD; 4705 bp) was employed in an attempt to ascertain the taxonomy of this isolate. Based on this polyphasic approach, we describe a novel species of the genus Aeromonas, for which the name Aeromonas cavernicola sp. nov. is proposed, with strain CCM7641T (DSM24474T, CECT7862T) as the type strain.
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Financial support from the Ministry of Education, Youth and Sports of the Czech Republic (project MSM0021622416) is acknowledged.
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The GenBank accession numbers of strain CCM7 641T for the 16S rRNA, gyrB, rpoD, recA, dnaJ, gyrA, dnaX and atpD genes sequences are HQ436040, HQ442702, HQ442864, HQ442978, HQ443080, HQ443164, HQ442426 and HQ442573, respectively.
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Martínez-Murcia, A., Beaz-Hidalgo, R., Svec, P. et al. Aeromonas cavernicola sp. nov., isolated from fresh water of a brook in a cavern. Curr Microbiol 66, 197–204 (2013). https://doi.org/10.1007/s00284-012-0253-x
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DOI: https://doi.org/10.1007/s00284-012-0253-x