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Current Microbiology

, Volume 64, Issue 5, pp 457–462 | Cite as

Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C

  • Majed M. MasadehEmail author
  • Nizar M. Mhaidat
  • Karem H. Alzoubi
  • Sayer I. Al-Azzam
  • Ashraf I. Shaweesh
Article

Abstract

In the present study, we investigated the possible involvement of oxidative stress in ciprofloxacin-induced cytotoxicity against several reference bacteria including Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 29213, and clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA). Oxidative stress was assessed by measurement of hydrogen peroxide generation using a FACScan flow cytometer. The antibacterial activity of ciprofloxacin was assessed using the disk diffusion method and by measuring the minimum inhibitory concentration (MIC). Ciprofloxacin induced a dose-dependent antibacterial activity against all bacteria where the highest tested concentration was 100 ug/ml. Results revealed that E. coli cells were highly sensitive to ciprofloxacin (MIC = 0.21 μg/mL ± 0.087), P. aeruginosa and S. aureus cells were intermediately sensitive (MIC = 5.40 μg/mL ± 0.14; MIC = 3.42 μg/mL ± 0.377, respectively), and MRSA cells were highly resistant (MIC = 16.76 μg/mL ± 2.1). Pretreatment of E. coli cells with either vitamin E or vitamin C has significantly protected cells against ciprofloxacin-induced cytotoxicity. These results indicate the possible antagonistic properties for vitamins C or E when they are used concurrently with ciprofloxacin.

Keywords

Reactive Oxygen Species Minimum Inhibitory Concentration Quinolones Enrofloxacin Dichlorofluorescein Diacetate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

The authors would like to acknowledge Jordan University of Science & Technology, Irbid; Jordan, for their financial support (grant number 73-2006).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Majed M. Masadeh
    • 1
    Email author
  • Nizar M. Mhaidat
    • 1
  • Karem H. Alzoubi
    • 1
  • Sayer I. Al-Azzam
    • 1
  • Ashraf I. Shaweesh
    • 2
  1. 1.Faculty of PharmacyJordan University of Science & TechnologyIrbidJordan
  2. 2.Faculty of DentistryJordan University of Science & TechnologyIrbidJordan

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