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Leucobacter margaritiformis sp. nov., Isolated from Bamboo Extract

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Abstract

A Gram-positive aerobic rod-shaped non-motile bacterium designated A23T was isolated from bamboo extract that had been used to remove odor and was characterized to determine its taxonomic position. 16S rRNA gene sequence analysis revealed that strain A23T belongs to the phylum Actinobacteria. The highest degree of sequence similarities was determined to be with Leucobacter salsicius M1-8T (96.7%), Leucobacter exalbidus K-540BT (96.4%), Leucobacter chromiireducens subsp. chromiireducens L-1T (96.4%), Leucobacter komagatae IFO 15245T (96.4%) and Leucobacter aerolatus Sj10T (96.4%). Chemotaxonomic data revealed that strain A23T possesses menaquinone MK11, and its cell wall peptidoglycan contained 2,4-diaminobutyric acid, alanine, glycine, glutamic acid and γ-aminobutyric acid. The polar lipid profile of strain A23T contained diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The predominant fatty acids were iso-C16:0 (31.5%), anteiso-C15:0 (43.2%) and anteiso-C17:0 (13.9%), all of which corroborated the assignment of the strain to the genus Leucobacter. Based on these data, A23T (=KEMC 551-022T = JCM 17538T) should be classified as the type strain for a novel Leucobacter species, for which the name Leucobacter margaritiformis sp. nov. is proposed.

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Acknowledgments

This research work was supported by Korea Ministry of Environment as: The GAIA Project (173-092-012) and Korea Ministry of Educational Science and Technology (2011-0000544).

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Authors and Affiliations

  1. Division of Natural Science, Department of Bioengineering, Kyonggi University, 94-6 Iui-dong, Yeongtong-gu, Suwon, 433-760, Republic of Korea

    Jin-Ha Lee

  2. Division of Natural Science, Department of Life Science, Kyonggi University, 94-6 Iui-dong, Yeongtong-gu, Suwon, 433-760, Republic of Korea

    Sang-Seob Lee

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  1. Jin-Ha Lee
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Correspondence to Sang-Seob Lee.

Additional information

The NCBI GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain A23T is JN038197.

Electronic supplementary material

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284_2012_89_MOESM1_ESM.ppt

Supplementary Fig. S1. Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, showing the phylogenetic relationships between strain A23T and related species (all Leucobacter species and related genera). Bar represents 20 substitutions per nucleotide position. Microbial names in bold indicate type species of the related genera. (PPT 182 kb)

284_2012_89_MOESM2_ESM.ppt

Supplementary Fig. S2. Maximum-likelihood phylogenetic tree based on 16S rRNA gene sequences, showing the phylogenetic relationships between strain A23T and related species (all Leucobacter species and related genera). Bar represents 0.02 substitutions per nucleotide position. Microbial names in bold indicate type species of the related genera. (PPT 177 kb)

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Lee, JH., Lee, SS. Leucobacter margaritiformis sp. nov., Isolated from Bamboo Extract. Curr Microbiol 64, 441–448 (2012). https://doi.org/10.1007/s00284-012-0089-4

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  • DOI: https://doi.org/10.1007/s00284-012-0089-4

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