Abstract
The purpose of the present study was to determine the inhibitory activities of two bacteriocins, produced by lactobacilli, against genital mycoplasmas. In this study, infections produced by genital mycoplasmas were studied; of these, 1.3% were caused by Mycoplasma hominis, 10.7% by Ureaplasma urealyticum and 5.6% by U. urealyticum + M. hominis. U. urealyticum was isolated from 75 out of 123 patients with genital mycoplasmas, while M. hominis was isolated from 9 patients (7.3%) and both U. urealyticum and M. hominis from 39 patients (31.7%). Bacteriocins, L23 and L60, produced by Lactobacillus fermentum and L. rhamnosus, respectively, appear to be two novel inhibitors of bacterial infection with potential antibacterial activity. Both bacteriocins proved to be active against 100% of strains tested; MICs of bacteriocin L23 ranged between 320 and 160 UA ml−1 for 78% of the M. hominis strains and between 320 and 80 UA ml−1 for 95% of the U. urealyticum strains. In addition, bacteriocin L60 was still active at 160 UA ml−1 for a high percentage (56%) of M. hominis strains, and at 80 UA ml−1 for 53% of the U. urealyticum strains. Interestingly, these antimicrobial substances produced by lactobacilli showed an inhibitory activity against genital mycoplasmas even when diluted. Altogether, our study indicates that the bacteriocins, L23 and L60, are good candidates for the treatment or prevention of genital infections in women.
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References
Ali D, Lacroix C, Thuault D, Bourgeois C, Simard R (1995) Characterization of diacetin B, a bacteriocin from Lactococcus lactis subsp. lactis bv. diacetylactis UL720. Can J Microbiol 41:832–841
Ardic N, Oncul O, Ilga U, Turhan V, Haznedaroglu T, Ozyurt M (2005) Investigation of the frequency and antibiotic susceptibility of Mycoplasma/Ureaplasma in urine samples with leukocyturia by different commercial methods. Int Infect Dis 4:2. www.ispub.com
Atassi F, Brassart D, Grob P, Graf F, Servin A (2006) Lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit Prevotella bivia and Gardnerella vaginalis in coculture and cell culture. FEMS Immunol Med Microbiol 48(3):424–432
Bayraktar MR, Ozerol IH, Gucluer N, Celik O (2010) Prevalence and antibiotic susceptibility of Mycoplasma hominis and Ureaplasma urealyticum in pregnant women. Int J Infect Dis 14(2):90–95
Benoit V, Mathis R, Lefebvre G (1996) Characterization of Brevicin 27, a bacteriocin synthesized by Lactobacillus brevis SB27. Curr Microbiol 28:53–61
Bergey (1994) Manual of determinative bacteriology, ninth edition. 19:565–570
Boris S, Barbes C (2000) Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes Infect 2:543–546
Czaja C, Stapleton A, Yarrova-Yarovaya Y Stamm W (2007) Phase I trial of a Lactobacillus crispatus vaginal suppository for prevention of recurrent urinary tract infection in women. Infect Dis Obstet Gynecol 2007:35387
De Man JC, Rogosa M, Sharp ME (1960) A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135
Elias M, Grzesko J, Siejkowski R, Nowicka J, Maczynska B, Goluda M, St Gabrys M (2005) The presence of Mycoplasma hominis and Ureaplasma urealyticum in the cervical canal of uterus. Ginekol Pol 76(1):28–32
Fagundo-Sierra R, Sanchez-Saínz A, Pérez-Jáuregui J (2006) Resistencia in vitro de aislamientos clínicos de Mycoplasma hominis y Ureaplasma urealyticum en México. Bioquimia 31(4):124–131
Gdoura R, Kchaou W, Chaari C, Znazen A, Keskes L, Rebai T Hammami A (2007) Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis and Mycoplasma genitalium infections and semen quality of infertile men. BMC Infect Dis 7:129. doi:10.1186/1471-2334-7-129
Gokahmetoglu S, Özçelik B, Kutukolu I, Saatç E, Özbal Y (2004) Vajinal Akintisi Olan Kadinlarda Mycoplasma hominis ve Ureaplasma urealyticum Sikligini ve Antimikrobiyallere Direnç Durumunun Belirlenmesi. Artemio 5(2):159–161
Hutt P, Shchepetova J, Livukene K, Mikelsaar M (2006) Antagonistic activity of probiotic lactobacilli and bifidobacteria against entero and uropathogens. J Appl Microbiol 100:1324–1332
Karabay O, Topcuoglu A, Kocoglu E, Gurel S, Gurel H, Ince NK (2006) Prevalence and antibiotic susceptibility of genital Mycoplasma hominis and Ureaplasma urealyticum in a university hospital in Turkey. Clin Exp Obstet Gynecol 33(1):36–38
Kilic D, Basar M, Kaygusuz S, Yilmaz E, Basar H, Batislam E (2004) Prevalence and treatment of Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis in patients with non-gonococcal urethritis. Jpn J Infect Dis 57:17–20
Nassar FA, Abu-Elamreen FH, Shubair ME, Sharif FA (2008) Detection of Chlamydia trachomatis and Mycoplasma hominis, M. genitalium and Ureaplasma urealyticum by polymerase chain reaction in patients with sterile pyuria. Adv Med Sci 53(1):80–86
Pascual L (2004) Bacteriocinogenia en el género Lactobacillus: características benéficas de lactobacilos de vagina humana. Tesis doctoral. Universidad Nacional de Río Cuarto, Río Cuarto
Pascual L, Daniele MB, Pájaro C, Barberis L (2006) Lactobacillus species isolated from the vagina: identification, hydrogen, peroxide production and nonoxynol-9 resistance. Contraception 73(1):78–81
Pascual L, Daniele MB, Giordano W, Barberis L (2008) Purification and partial characterization of novel bacteriocin L23 produced by Lactobacillus fermentum L23. Curr Microbiol 56:397–402
Pascual L, Daniele MB, Giordano W, Barberis L (2008) Lactobacillus rhamnosus L60 a potencial probiotic isolated from human vagina. J Gen Appl Microbiol 54(3):141–148
Pereyre S, Renaudin H, Charron A, Bébéar C, Bébéar CM (2006) Emergence of a 23S rRNA mutation in Mycoplasma hominis associated with a loss of the intrinsic resistance to erythromycin and azithromycin. J Antimicrob Chemother 57(4):753–756
Ramírez-Isarraraz C, Casanova RG, Menocal T, Ortiz-Ibarra FY, Ahued–Ahued R (2004) Prevalencia de la infección cervicovaginal por Mycoplasma hominis y Ureaplasma urealyticum en pacientes ginecológicas del Instituto Nacional de Perinatología. Enf Inf Microbiol 24(1):1–5
Reid G, Burton J (2002) Use of Lactobacillus to prevent infection by pathogenic bacteria. Microbes Infect 4(3):319–324
Reid G (2008) Probiotic lactobacilli for urogenital health in women. J Clin Gastroenterol 2:234–236
Roca A (2006) Infecciones por mycoplasmas. Rev Clin Esp 206(5):239–242
Ruiz F, Gerbaldo G, Asurmendi P, Pascual L, Giordano W, Barberis L (2009) Antimicrobial activity, inhibition of urogenital pathogens, and synergistic interactions between lactobacillus strains. Curr Microbiol 59:497–501
Tiodorović J, Randelović G, Kocić B, Tiodorović-Zivković D (2007) Bacteriological finding in the urethra in men with and without non-gonococcal urethritis. Vojnosanit Pregl 64(12):833–836
Zhou CX, Huang JH, Chen J, Lu JY, Xiang YP (2006) Female urogenital mycoplasma infection and drug sensitivity status in Changsha. Nan Fang Yi Ke Da Xue Xue Bao 26(6):831–836
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This study was supported by the Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto.
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Daniele, M., Ruiz, F., Pascual, L. et al. Ureaplasma urealyticum and Mycoplasma hominis Sensitivity to Bacteriocins Produced by Two Lactobacilli Strains. Curr Microbiol 63, 360 (2011). https://doi.org/10.1007/s00284-011-9989-y
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DOI: https://doi.org/10.1007/s00284-011-9989-y