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Cytotoxic Activity of Fungal Metabolites from the Pathogenic Fungus Beauveria bassiana: An Intraspecific Evaluation of Beauvericin Production

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Abstract

The cyclohexadepsipeptide beauvericin (BEA) is a mycotoxin produced by the fungus Beauveria bassiana (Bals.). Using ELISA, different accessions of B. bassiana, belonging to distinct genetic groups, were analyzed to determine their variability in BEA production. The cytotoxic effect of pure mycotoxins and crude extracts was also tested on insect cell lines SF-9 and SF-21. The results showed that BEA production was significantly different between all strains. Bb 9024 exhibited the highest levels (98.56 mg/l), while Bb 9001 the lowest (15.66 mg/l). Statistical difference was found when BEA CC50 values (2.81 and 6.94 μM) were compared with those values from others mycotoxins (4.23–11.95 μM). Although no correlation has been observed between beauvericin production and phylogenetic grouping, the results suggest a comprehensible involvement of these metabolites during the infection process. The biological evaluation of metabolites produce by entomopathogenic fungi provides better criteria to design more effective formulations for pest management.

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Acknowledgments

The work was supported by Colombian Institute for development of Science and Technology “Francisco José de Caldas” COLCIENCIAS and National Federation of Coffee Growers of Colombia by its National Coffee Research Center, CENICAFE. We want also to thank the Coordination of Improvement of Higher Education, CAPES, Brazil, for the support given to JWAV (First Author). Additionally, the authors thank to the Laboratory of virology at CENARGEN for training given to JWAV and Universidad Católica de Manizales—UCM (Colombia) for the support received in their laboratories.

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Authors and Affiliations

  1. Centro Nacional de Investigaciones de Café, CENICAFE-FEDERACAFE, Disciplina de Fitopatologia, Planalto Km 4 Via a Manizales, Chinchiná, Caldas, Colombia

    Jorge W. Arboleda Valencia & Alvaro L. Gaitán Bustamante

  2. Departamento de Biologia Celular, Universidade de Brasília, Asa Norte, Brasília, DF, 70910-900, Brazil

    Jorge W. Arboleda Valencia

  3. Grupo de Investigaciones Plátano, Departamento de Fitotecnia, Facultad de Ciencias Agropecuarias, Universidad de Caldas, Calle 65 # 26-10, Manizales, Caldas, Colombia

    Arnubio Valencia Jiménez

  4. Embrapa Recursos Genéticos e Biotecnologia, PqEB Final W5 Norte, CP 02372, Asa Norte, Brasília, DF, 70770-900, Brazil

    Jorge W. Arboleda Valencia & Ma Fátima Grossi-de-Sá

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  1. Jorge W. Arboleda Valencia
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  2. Alvaro L. Gaitán Bustamante
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  3. Arnubio Valencia Jiménez
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  4. Ma Fátima Grossi-de-Sá
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Correspondence to Jorge W. Arboleda Valencia.

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Valencia, J.W.A., Gaitán Bustamante, A.L., Jiménez, A.V. et al. Cytotoxic Activity of Fungal Metabolites from the Pathogenic Fungus Beauveria bassiana: An Intraspecific Evaluation of Beauvericin Production. Curr Microbiol 63, 306–312 (2011). https://doi.org/10.1007/s00284-011-9977-2

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