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Actimicrobium antarcticum gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater


A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic, and short rod-shaped bacterium that was designated strain KOPRI 25157T was isolated from coastal seawater sample in Antarctica. The temperature and pH ranges for growth on R2A agar were 10–20°C, and 5.0–10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157T showed it to belong to the family Oxalobacteraceae of the class Betaproteobacteria, and it formed a distinct clade from other recognized members of the family. DNA G + C content was 65.9 mol%. Major ubiquinone was Q-8. Predominant cellular fatty acids were C16:1 ω7c/15 iso 2OH (56.4%) and C16:1 (30.5%). Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and unknown lipid. On the basis of these data, it is proposed that strain KOPRI 25157T is the representative of a novel genus, for which the name Actimicrobium gen. nov. is proposed in the family Oxalobacteraceae. The type strain for Actimicrobium antarcticum sp. nov. is KOPRI 25157T (=JCM 16673T=KCTC 23040T).

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We are grateful to Dr. J. P. Euzéby for help with nomenclature. This study was supported by Korea Polar Research Institute (Grant PE10050).

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Correspondence to Soon Gyu Hong.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain KOPRI 25157T is HQ699437.

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Fig. S1 Two-dimensional thin-layer chromatogram of polar lipids of strain KOPRI 25157T. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; AL, unknown aminolipids; L, unknown polar lipid. (PDF 19 kb)

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Kim, E.H., Jeong, HJ., Lee, Y.K. et al. Actimicrobium antarcticum gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater. Curr Microbiol 63, 213–217 (2011).

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  • Betaproteobacteria
  • Isoprenoid Quinone
  • Major Polar Lipid
  • KCTC 23040T
  • Burkholderiales