Skip to main content

Actimicrobium antarcticum gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater

Abstract

A Gram-negative, non-motile, catalase- and oxidase- positive, strictly aerobic, and short rod-shaped bacterium that was designated strain KOPRI 25157T was isolated from coastal seawater sample in Antarctica. The temperature and pH ranges for growth on R2A agar were 10–20°C, and 5.0–10.0, respectively. Phylogenetic analyses of the 16S rRNA gene sequence of strain KOPRI 25157T showed it to belong to the family Oxalobacteraceae of the class Betaproteobacteria, and it formed a distinct clade from other recognized members of the family. DNA G + C content was 65.9 mol%. Major ubiquinone was Q-8. Predominant cellular fatty acids were C16:1 ω7c/15 iso 2OH (56.4%) and C16:1 (30.5%). Major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, and unknown lipid. On the basis of these data, it is proposed that strain KOPRI 25157T is the representative of a novel genus, for which the name Actimicrobium gen. nov. is proposed in the family Oxalobacteraceae. The type strain for Actimicrobium antarcticum sp. nov. is KOPRI 25157T (=JCM 16673T=KCTC 23040T).

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2

References

  1. Bowman JP, Sly LI, Hayward AC, Spiegel LY, Stackebrandt E (1993) Telluria mixta (Pseudomonas mixta Bowman, Sly, and Hayward 1988) gen. nov., comb. nov, and Telluria chitinolytica sp. nov., soil-dwelling organisms which actively degrade polysaccharides. Int J Syst Bacteriol 43:120–124

    PubMed  Article  CAS  Google Scholar 

  2. Choi A, Oh HM, Cho JC (2011) Saccharospirillum aestuarii sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol (in press)

  3. Chun J, Lee JH, Jung Y, Kim M, Kim S, Kim BK, Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    PubMed  Article  CAS  Google Scholar 

  4. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376

    PubMed  Article  CAS  Google Scholar 

  5. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  6. Fernandes C, Rainey FA, Nobre MF, Pinhal I, Folhas F, Costa MS (2005) Herminiimonas fonticola gen. nov., sp. nov., a Betaproteobacterium isolated from a source of bottled mineral water. Syst Appl Microbiol 28:596–603

    PubMed  Article  CAS  Google Scholar 

  7. Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416

    Article  Google Scholar 

  8. Garrity GM, Winters M, Searles DB (2001) Taxonomic outline of the procaryotic genera. In: Bergey’s manual of systematic bacteriology, 2nd edn. online release 1.0, Springer, New York. (http://www.cme.msu.edu/bergeys/april2001-genus.pdf)

  9. Hiraishi A, Shin YK, Sugiyama J (1997) Proposal to reclassify Zoogloea ramigera IAM 12670 (P. R. Dugan 115) as Duganella zoogloeoides gen. nov., sp. nov. Int J Syst Bacteriol 47:1249–1252

    PubMed  Article  CAS  Google Scholar 

  10. Kämpfer P, Falsen E, Busse HJ (2008) Naxibacter varians sp. nov. and Naxibacter haematophilus sp. nov., and emended description of the genus Naxibacter. Int J Syst Evol Microbiol 58:1680–1684

    PubMed  Article  Google Scholar 

  11. Kämpfer P, Rosselló-Mora R, Hermansson M, Persson F, Huber B, Falsen E, Busse HJ (2007) Undibacterium pigrum gen. nov., sp. nov., isolated from drinking water. Int J Syst Evol Microbiol 57:1510–1515

    PubMed  Article  Google Scholar 

  12. Kimura M (1980) A sample method for estimation evolutionary rate of base substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    PubMed  Article  CAS  Google Scholar 

  13. Komagata K, Suzuki KI (1987) Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–206

    Article  CAS  Google Scholar 

  14. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. John Wiley, New York, pp 115–175

    Google Scholar 

  15. Lee YK, Jung HJ, Lee HK (2006) Marine bacteria associated with the Korean brown alga, Undaria pinnatifida. J Microbiol 44:694–698

    PubMed  CAS  Google Scholar 

  16. Li WJ, Zhang YQ, Park DJ, Li CT, Xu LH, Kim CJ, Jiang CL (2004) Duganella violaceinigra sp. Nov., a novel mesophilic bacterium isolated from forest soil. Int J Syst Evol Microbiol 54:1811–1814

    PubMed  Article  CAS  Google Scholar 

  17. Lincoln SP, Fermor TR, Tindall BJ (1999) Janthinobacterium agaricidamnosum sp. nov., a soft rot pathogen of Agaricus bisporus. Int J Syst Bacteriol 49:1577–1589

    PubMed  Article  CAS  Google Scholar 

  18. Loveland-Curtze J, Miteva VI, Brenchley JE (2009) Herminiimonas glaciei sp. nov., a novel ultramicrobacterium from 3042 m deep Greenland glacial ice. Int J Syst Evol Microbiol 59:1272–1277

    PubMed  Article  CAS  Google Scholar 

  19. Mesbah M, Premachandran U, Whiman WB (1989) Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167

    Article  CAS  Google Scholar 

  20. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241

    Article  CAS  Google Scholar 

  21. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  22. Scola BL, Birtles RJ, Mallet MN, Raoult D (1998) Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions. J Clin Microbiol 36:2847–2852

    PubMed  Google Scholar 

  23. Swofford D (2002) PAUP: phylogenetic analysis using parsimony (and other methods). Sinauer Associates, Sunderland

  24. Weon HY, Kim BY, Son JA, Jang HB, Hong SK, Go SJ, Kwon SW (2008) Massilia aerilate sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 58:1422–1425

    PubMed  Article  CAS  Google Scholar 

  25. Xu P, Li WJ, Tang SK, Zhang YQ, Chen GZ, Chen HH, Xu LH, Jiang CL (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153

    PubMed  Article  CAS  Google Scholar 

  26. Yamada Y (1998) Identification of coenzyme Q (ubiquinone) homologs. In: Kurtzman CP, Fell JW (eds) The yeasts–a taxonomic study. Elsevier, Amsterdam, pp 101–102

    Google Scholar 

  27. Yokota A, Akagawa-Matsushita M, Hiraishi A, Katayama Y, Urakami T, Yamasato K (1992) Distribution of quinone systems in microorganisms: gram-negative eubacteria. Bull Jpn Fed Cult Coll 8:136–171

    Google Scholar 

Download references

Acknowledgments

We are grateful to Dr. J. P. Euzéby for help with nomenclature. This study was supported by Korea Polar Research Institute (Grant PE10050).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Soon Gyu Hong.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain KOPRI 25157T is HQ699437.

Electronic supplementary material

Below is the link to the electronic supplementary material.

284_2011_9962_MOESM1_ESM.pdf

Fig. S1 Two-dimensional thin-layer chromatogram of polar lipids of strain KOPRI 25157T. DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine; AL, unknown aminolipids; L, unknown polar lipid. (PDF 19 kb)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kim, E.H., Jeong, HJ., Lee, Y.K. et al. Actimicrobium antarcticum gen. nov., sp. nov., of the Family Oxalobacteraceae, Isolated from Antarctic Coastal Seawater. Curr Microbiol 63, 213–217 (2011). https://doi.org/10.1007/s00284-011-9962-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-011-9962-9

Keywords

  • Betaproteobacteria
  • Isoprenoid Quinone
  • Major Polar Lipid
  • KCTC 23040T
  • Burkholderiales