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Streptomyces autolyticus JX-47 Large-Insert Bacterial Artificial Chromosome Library Construction and Identification of Clones Covering Geldanamycin Biosynthesis Gene Cluster

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Abstract

Geldanamycin belongs to benzoquinone ansamycin antibiotic and has potent antitumor activities. In this study, a bacterial artificial chromosome (BAC) library with an average insert size of up to 150 kb was constructed from genomic DNA of Streptomyces autolyticus JX-47. A genetic-screening strategy was established using BAC end-sequencing and three pairs of primers designed to target the remote regions, gdmA1, gdmA3 and gdmRI, of the geldanamycin gene cluster. Three clones covering geldanamycin biosynthesis gene cluster were obtained, which together spanned a 250-kb genomic region, and a 150227-bp insert in the clone p4E9 was sequenced. Comparison with the reported geldanamycin gene cluster sequences from S. hygroscopicus revealed that it had the same gene arrangement and high gene homology in the polyketide synthase (PKS) region and its downstream with 84–100% DNA identity and 81–100% amino acid (AA) identity. Its DNA homology with the whole gene cluster sequence from S. hygroscopicus strain 17997 reached 99% identity. However, upstream of the PKS region exhibited great diversity, where only ORF16 was conserved, and the other genes including gdmL and gdmX were displaced.

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Acknowledgments

The authors indebted to Professor Chenglin Jiang and Lihua Xu, at the Key Laboratory for Microbial Resources of Ministry of Education, in Kunming, P.R. China for enabling the presentation of S. autolyticus JX-47. This study has been partially supported by a grant from China Ocean Mineral Resources R&D Association (DYXM115-02-2-14) and the State Principal and Basic Research and Development Program of the Ministry of Sciences and Technology of China (2010CB833801). The authors also thank the financial support by the Open Project Program (LMB091001) of the Key Laboratory of Marine Bio-resources Sustainable Utilization, SCSIO, CAS.

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Authors and Affiliations

  1. Key Laboratory of Marine Bio-Resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, People’s Republic of China

    Shikun Dai, Guanghua Wang & Xiang Li

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Shikun Dai

  3. Guangzhou Medical College, Guangzhou, 510182, People’s Republic of China

    Yongchang Ouyang

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  1. Shikun Dai
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  2. Yongchang Ouyang
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  3. Guanghua Wang
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  4. Xiang Li
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Correspondence to Xiang Li.

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Dai, S., Ouyang, Y., Wang, G. et al. Streptomyces autolyticus JX-47 Large-Insert Bacterial Artificial Chromosome Library Construction and Identification of Clones Covering Geldanamycin Biosynthesis Gene Cluster. Curr Microbiol 63, 68–74 (2011). https://doi.org/10.1007/s00284-011-9940-2

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  • DOI: https://doi.org/10.1007/s00284-011-9940-2

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