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A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants

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Abstract

In this study, bacteria hosted in root nodules of single plants of legume Arachis hypogaea L. (peanut) cv Tegua Runner growing at field were isolated. The collection of nodule isolates included both fast and slow growing strains. Their genetic diversity was assessed in order to identify the more frequently rhizobial strain associated to nodules from single plants. Molecular fingerprinting of 213 nodular isolates indicated heterogeneity, absence of a dominant genotype and, therefore, of a unique strains highly competitive. Efficient nitrogen-fixing isolates were identified as Bradyrhizobium sp. by phylogenetic analysis of the sequences of their 16S rRNA genes. The genetic diversity of 68 peanut nodulating isolates from all the collected plants was also analyzed. Considering their ERIC-PCR profiles, they were grouped in eighteen different OTUs for 60% similarity cut-off. Results obtained in this study indicate that the genetic diversity of rhizobia occupying nodules from single plant is very high, without the presence of a dominant strain. Therefore, the identification of useful peanut rhizobia for agricultural purposes requires strongly the selection, among the diverse population, of a very competitive genotype in combination with a high-symbiotic performance.

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Acknowledgments

This study was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Secretaría de Ciencia y Técnica de la Universidad Nacional de Río Cuarto (SECYT-UNRC), CONICET and Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), M. Tonelli and F. Ibañez are fellowships from CONICET, T. Taurian, J. Angelini and A. Fabra are members of research career of CONICET, Argentina.

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Authors and Affiliations

  1. Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Enlace Rutas 8 y 36 km 601, 5800, Río Cuarto, Córdoba, Argentina

    Jorge Angelini, Fernando Ibáñez, Tania Taurian, María Laura Tonelli, Lucio Valetti & Adriana Fabra

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  1. Jorge Angelini
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  2. Fernando Ibáñez
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  3. Tania Taurian
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  4. María Laura Tonelli
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  5. Lucio Valetti
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  6. Adriana Fabra
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Correspondence to Jorge Angelini.

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Angelini, J., Ibáñez, F., Taurian, T. et al. A Study on the Prevalence of Bacteria that Occupy Nodules within Single Peanut Plants. Curr Microbiol 62, 1752–1759 (2011). https://doi.org/10.1007/s00284-011-9924-2

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  • DOI: https://doi.org/10.1007/s00284-011-9924-2

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