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Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by Ancylobacter sp. XJ-412-1 Isolated from Soil

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Abstract

Ancylobacter sp. XJ-412-1, capable of degrading metsulfuron-methyl, was isolated from sulfonylurea-contaminated soil. When metsulfuron-methyl was provided as the sole carbon source, more than 90.5% of metsulfuron-methyl at concentration of 50 mg l−1 was degraded by strain XJ-412-1 after incubation at 30°C for 7 days. The initial degradation products of metsulfuron-methyl (MSM), thifensulfuron-methyl (TSM), and bensulfuron-methyl (BSM) by XJ-412-1 were identified as corresponding deesterified derivatives by liquid chromatography-mass spectrometry, which indicated a primary pathway of the deesterification of these three sulfonylurea herbicides. The carboxyesterase activity of the cell-free extracts was assayed and strongly inhibited by 4-chloromercuribenzoic acid (PCMB), diethyl pyrocarbonate (DEPC), phenylmethylsulfonyl fluoride (PMSF), and malathion.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30900044, 30830001), Fund for the Doctoral Program of Higher Education (20090097120031), Fund for SATAS (2010hzsZDZXZ001).

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Authors and Affiliations

  1. Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, People’s Republic of China

    Peng Lu, Lei Jin, Bin Liang, Jing Zhang, Shunpeng Li, Zhaozhong Feng & Xing Huang

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  1. Peng Lu
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  2. Lei Jin
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  3. Bin Liang
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  4. Jing Zhang
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  5. Shunpeng Li
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  6. Zhaozhong Feng
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  7. Xing Huang
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Correspondence to Xing Huang.

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Lu, P., Jin, L., Liang, B. et al. Study of Biochemical Pathway and Enzyme Involved in Metsulfuron-Methyl Degradation by Ancylobacter sp. XJ-412-1 Isolated from Soil. Curr Microbiol 62, 1718–1725 (2011). https://doi.org/10.1007/s00284-011-9919-z

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