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Characterization of Fluoroglycofen Ethyl Degradation by Strain Mycobacterium phocaicum MBWY-1

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Abstract

A fluoroglycofen ethyl-degrading bacterium, MBWY-1, was isolated from the soil of an herbicide factory. This isolated strain was identified as Mycobacterium phocaicum based on analysis of its 16S rRNA gene sequence and its morphological, physiological, and biochemical properties. The strain was able to utilize fluoroglycofen ethyl as its sole source of carbon for growth and could degrade 100 mg l−1 of fluoroglycofen ethyl to a non-detectable level within 72 h. The optimum temperature and pH for fluoroglycofen ethyl degradation by strain MBWY-1 were 30°C and 7.0, respectively. Five metabolites produced during the degradation of fluoroglycofen ethyl and were identified by mass spectrometry as {5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrophenylacyl} hydroxyacetic acid, acifluorfen, 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoate, 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-hydroxyl, and 3-chloro-4-hydroxyl benzotrifluoride. Identification of the metabolites allowed to propose the degradation pathway of fluoroglycofen ethyl by strain MBWY-1. The inoculation of strain MBWY-1 into soil treated with fluoroglycofen ethyl resulted in a higher fluoroglycofen ethyl degradation rate than in uninoculated soil regardless of whether the soil was sterilized or nonsterilized.

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Acknowledgments

We gratefully acknowledge Dr. Shichao Xu of the Nanjing Science and Technology University for excellent assistance with MS analysis. This study was supported by the Provincial Environmental Protection Scientific Research Projects of Jiangsu Provience (2009001) and National Technology Support Project (2008BAD96B05).

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  1. The College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Liwei Chen, Tianming Cai & Qingling Wang

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  1. Liwei Chen
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  2. Tianming Cai
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  3. Qingling Wang
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Correspondence to Tianming Cai.

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Chen, L., Cai, T. & Wang, Q. Characterization of Fluoroglycofen Ethyl Degradation by Strain Mycobacterium phocaicum MBWY-1. Curr Microbiol 62, 1710–1717 (2011). https://doi.org/10.1007/s00284-011-9918-0

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  • DOI: https://doi.org/10.1007/s00284-011-9918-0

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