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Prevalence of the bla SHV Gene in Klebsiella pneumoniae Isolates Obtained from Hospital and Community Infections and from the Microbiota of Healthy Individuals in Recife, Brazil

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Abstract

The aim of this study was to determine the prevalence of the bla SHV gene in Klebsiella pneumoniae isolates from hospital and community infections and from the normal microbiota of healthy individuals in Recife, PE, Brazil. Fifty-two K. pneumoniae isolates were analyzed regarding the presence of the bla SHV gene, using PCR, and eight isolates were analyzed by DNA sequencing. This gene was detected in 16 isolates from hospital infections, four from community infections, and nine from the normal microbiota. This was the first study to find the bla SHV gene in K. pneumoniae isolates from the normal microbiota. Through DNA sequencing of eight K. pneumoniae isolates from hospital and community infections, with a resistance phenotype indicative of extended-spectrum β-lactamase production, a new SHV variant named SHV-122 was found. We also detected the presence of bla SHV-1, bla SHV-11, bla SHV-28, and bla SHV-108. The results show that in Recife, Brazil, K. pneumoniae isolates that presented resistance to oxyimino-β-lactams had high prevalence and diversity of the bla SHV gene. We also conclude that there was a high presence of the bla SHV gene among isolates from the normal microbiota of healthy individuals.

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Acknowledgments

We are grateful to Dr. Vera Magalhães for supplying the community isolates and to Michel Satya Naslavsky for assistance during sequencing experiments. This study was financially supported by FACEPE (Fundação de Amparo à Pesquisa do Estado de Pernambuco).

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Authors and Affiliations

  1. Keizo Asami Immunopathology Laboratory, Federal University of Pernambuco (LIKA/UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE, 50670-901, Brazil

    Dyana Leal Veras, Luiz Carlos Alves, Fábio André Brayner, Cíntia Renata Costa Rocha & Ana Catarina de Souza Lopes

  2. Department of Parasitology, Aggeu Magalhães Research Center (CPqAM-Fiocruz), Av. Professor Moraes Rego, s/n Cidade Universitária, Recife, PE, 50.670-420, Brazil

    Dyana Leal Veras, Luiz Carlos Alves & Fábio André Brayner

  3. Department of Entomology, Aggeu Magalhães Research Center (CPqAM-Fiocruz), Av. Professor Moraes Rego, s/n Cidade Universitária, Recife, PE, 50.670-420, Brazil

    Duschinka Ribeiro Duarte Guedes

  4. Department of Tropical Medicine, Federal University of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE, 50670-901, Brazil

    Fábio André Brayner, Maria Amélia Vieira Maciel & Ana Catarina de Souza Lopes

  5. Department of Biochemistry, Federal University of Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235, Cidade Universitária, Recife, PE, 50670-901, Brazil

    Cíntia Renata Costa Rocha

  6. Centro de Pesquisas Aggeu Magalhães—CPqAM/Fiocruz, Av. Professor Moraes Rego, s/n. Cidade Universitária, Caixa Postal 7472, Recife, PE, 50.670-420, Brazil

    Dyana Leal Veras

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  1. Dyana Leal Veras
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  2. Luiz Carlos Alves
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  3. Fábio André Brayner
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  4. Duschinka Ribeiro Duarte Guedes
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  5. Maria Amélia Vieira Maciel
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  6. Cíntia Renata Costa Rocha
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  7. Ana Catarina de Souza Lopes
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Correspondence to Dyana Leal Veras.

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Veras, D.L., Alves, L.C., Brayner, F.A. et al. Prevalence of the bla SHV Gene in Klebsiella pneumoniae Isolates Obtained from Hospital and Community Infections and from the Microbiota of Healthy Individuals in Recife, Brazil. Curr Microbiol 62, 1610–1616 (2011). https://doi.org/10.1007/s00284-011-9899-z

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  • DOI: https://doi.org/10.1007/s00284-011-9899-z

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