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Immunogenicity of Orally Administrated Recombinant Lactobacillus casei Zhang Expressing Cryptosporidium parvum Surface Adhesion Protein P23 in Mice

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Abstract

Cryptosporidium parvum, an intestinal apicomplexan parasite, is a significant cause of diarrheal diseases in both humans and animals. What is more, there is no promising strategy for controlling cryptosporidiosis. In this study, the P23 immunodominant surface protein of C. parvum sporozoites was stably expressed in the Lactobacillus casei Zhang strain and its immunogenicity was evaluated in a mouse model. The molecular weight (23 kDa) and immunogenicity of p23 gene expressed by L. casei Zhang were similar to that of the native P23 protein. Oral immunization with control L. casei Zhang and recombinant L. casei Zhang-p23 activated the mucosal immune system to elicit serum immunoglobulin G (IgG) and mucosal IgA in mice. Furthermore, the expression of cytokines such as IL-4, IL-6, and IFN-γ in splenocytes of mice was detected by real-time PCR after oral immunization. P23-specific immunocyte activation was also verified. These findings indicate that the live L. casei Zhang vector may be a new tool for the production of mucosal vaccines against cryptosporidiosis in animals.

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Acknowledgments

This work was supported by China Natural Science Foundation (Grant No. 30960279) and the Postdoctoral Foundation of Inner Mongolia Agricultural University (No. 57545).

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Authors and Affiliations

  1. Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia, 010018, China

    Geriletu & Heping Zhang

  2. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China

    Rihua Xu

  3. National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokaido, Japan

    Honglin Jia, Mohamad Alaa Terkawi & Xuenan Xuan

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  1. Geriletu
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  2. Rihua Xu
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  3. Honglin Jia
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  4. Mohamad Alaa Terkawi
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  5. Xuenan Xuan
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  6. Heping Zhang
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Correspondence to Heping Zhang.

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Geriletu and Rihua Xu equally contributed to this work.

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Geriletu, Xu, R., Jia, H. et al. Immunogenicity of Orally Administrated Recombinant Lactobacillus casei Zhang Expressing Cryptosporidium parvum Surface Adhesion Protein P23 in Mice. Curr Microbiol 62, 1573–1580 (2011). https://doi.org/10.1007/s00284-011-9894-4

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  • DOI: https://doi.org/10.1007/s00284-011-9894-4

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