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Identification and Assessment of Symbiotic Effectiveness of Phage-Typed Rhizobium leguminosarum Strains on Lentil (Lens culinaris Medik) Cultivars

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Abstract

Symbiotic effectiveness of 19 indigenous and two exotic (USDA 2426 and USDA 2431) strains of lentil Rhizobium belonging to different phage-sensitive and phage-resistant groups was compared under axenic condition. Four strains (USDA 2431, BHULR 104, BHULR 113, and BHULR 115) sensitive to different phages were found significantly superior over others in terms of nodule number, acetylene reduction activity, and total dry weight per plant. Inoculation response of these strains was then evaluated on six lentil cultivars under field condition. A significant symbiotic interaction between rhizobial strains and lentil cultivars was observed. Grain yield enhancement was noticed by the compatible interaction of lentil cultivars HUL-57, L-4147, K-75, and PL-4/DPL-15/DPL-62 with rhizobial strains USDA 2431, BHULR 104, BHULR 113, and BHULR 115, respectively. The authentication of rhizobial strains was accomplished through 16S rDNA sequence analysis. All rhizobial strains had close matching with R. leguminosarum bv. viciae strains. The results have shown that phages can trustfully help selecting out the symbiotically efficient most rhizobial strains for advantageous use with lentil cultivars, in order to strengthen the BNF-based future lentil breeding programs.

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Acknowledgment

The authors thank Prof. A. Vaishampayan for suggestions in the manuscript. The financial assistance from Department of Biotechnology, New Delhi is sincerely acknowledged.

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  1. Department of Genetics and Plant Breeding, Microbiology Laboratory, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, 221005, India

    Sanjay Kumar Jaiswal & Banshi Dhar

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  1. Sanjay Kumar Jaiswal
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  2. Banshi Dhar
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Correspondence to Banshi Dhar.

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Jaiswal, S.K., Dhar, B. Identification and Assessment of Symbiotic Effectiveness of Phage-Typed Rhizobium leguminosarum Strains on Lentil (Lens culinaris Medik) Cultivars. Curr Microbiol 62, 1503–1509 (2011). https://doi.org/10.1007/s00284-011-9871-y

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  • DOI: https://doi.org/10.1007/s00284-011-9871-y

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