Abstract
Triazophos is one of the most widely used organophosphorus insecticides usually detectable in the environment. A bacterial strain, Diaphorobacter sp. TPD-1, capable of using triazophos and its intermediate, 1-phenyl-3-hydroxy-1,2,4-triazole (PHT), as its sole carbon sources for growth was isolated from a triazophos-contaminated soil in China. This strain could completely degrade 50 mg l−1 triazophos and PHT to non-detectable level in 24 and 56 h, respectively. During PHT degradation, three metabolites were detected and identified based on tandem mass spectrometry (MS/MS) analysis. Using this information, a biochemical degradation pathway of triazophos by Diaphorobacter sp. TPD-1 was proposed. The first step involved in the degradation of triazophos is the hydrolysis of the P–O ester bond of triazophos to form PHT and o,o-diethyl phosphorothioic acid, then the triazol ring of PHT is subsequently cleaved to form (E)-1-formyl-2-phenyldiazene. Subsequently, (E)-1-formyl-2-phenyldiazene is transformed to 2-phenylhydrazinecarboxylic acid by adding one molecular of H2O. Finally, the carboxyl group of 2-phenylhydrazinecarboxylic acid is decarboxylated to form phenylhydrazine.
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Acknowledgments
This work was supported by grants from the Major Projects on Control and Rectification of Water Body Pollution (2009ZX07103-002), the Chinese National Natural Science Foundation (31070100), and the Key Technology R&D Program of Jiangsu Province (BE2008669).
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Chenli Yang and Rong Li contributed equally to this article.
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Yang, C., Li, R., Song, Y. et al. Identification of the Biochemical Degradation Pathway of Triazophos and its Intermediate in Diaphorobacter sp. TPD-1. Curr Microbiol 62, 1294–1301 (2011). https://doi.org/10.1007/s00284-010-9859-z
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DOI: https://doi.org/10.1007/s00284-010-9859-z