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Important Role of Catalase in the Production of β-carotene by Recombinant Saccharomyces cerevisiae under H2O2 Stress

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Abstract

The effect of H2O2 supplement on cell growth and β-carotene productions in recombinant Saccharomyces cerevisiae CFW-01 and CFW-01 ctt1 deficiency in cytosolic catalase were investigated in shaking flasks. The results showed that supplement of H2O2 (0.5 and 1.0 mM) can significantly stimulate the β-carotene production. However, β-carotene levels of CFW-01 ctt1Δ under 0.5 and 1 mM H2O2 were 16.7 and 36.7% lower than those of CFW-01, respectively. Although lacking cytosolic catalase, no significant differences in cell growth were observed between CFW-01 ctt1Δ and CFW-01 under the same level of H2O2 stress. These results suggest that β-carotene can act as an antioxidant to protect the recombinant yeast from H2O2 oxidative damage in the absence of cytosolic catalase. However, catalase still plays an important role in the production of β-carotene under H2O2 stress. If catalase can not timely decompose H2O2, the free radicals such as OH· derived from H2O2 can result in decrease of β-carotene concentration. Therefore, in the production of β-carotene by H2O2 stress, not only the level of oxidative stress, but also the activities of catalase in cells should be considered.

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Acknowledgments

This research was supported by grants from the National High Technology Research and Development Program of China (863 Program) (No 2007AA10Z341) and National Natural Science Foundation of China (No 31000811/C200207) and Open Project Program of State Key Laboratory of Food Science and Technology in Jiangnan University. We also thank Dr Rene Verwaal in Netherland for his kindly providing the vector YIplac211 used in the experiment.

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Authors and Affiliations

  1. Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, People’s Republic of China

    Guo-liang Yan, Heng-yu Liang, Zhi-qun Wang, Xiao-fan Yang, Dan Liu & Chang-qing Duan

  2. Department of Food Science, Tianjin Agricultural University, Tianjin, 300384, People’s Republic of China

    Jin-fu Liu

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  1. Guo-liang Yan
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  2. Heng-yu Liang
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  5. Dan Liu
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  7. Chang-qing Duan
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Correspondence to Chang-qing Duan.

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Heng-yu Liang has equally contributed to this work

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Yan, Gl., Liang, Hy., Wang, Zq. et al. Important Role of Catalase in the Production of β-carotene by Recombinant Saccharomyces cerevisiae under H2O2 Stress. Curr Microbiol 62, 1056–1061 (2011). https://doi.org/10.1007/s00284-010-9826-8

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  • DOI: https://doi.org/10.1007/s00284-010-9826-8

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