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The Euryhaline Yeast Debaryomyces hansenii has Two Catalase Genes Encoding Enzymes with Differential Activity Profile

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Abstract

Debaryomyces hansenii is a spoilage yeast able to grow in a variety of ecological niches, from seawater to dairy products. Results presented in this article show that (i) D. hansenii has an inherent resistance to H2O2 which could be attributed to the fact that this yeast has a basal catalase activity which is several-fold higher than that observed in Saccharomyces cerevisiae under the same culture conditions, (ii) D. hansenii has two genes (DhCTA1 and DhCTT1) encoding two catalase isozymes with a differential enzymatic activity profile which is not strictly correlated with a differential expression profile of the encoding genes.

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Acknowledgments

The authors are deeply indebted with the advices of René Cárdenas throughout the work, of Facultad de Ciencias, UNAM and to the technical assistance of Cristina Aranda and Pablo Rangel, from Instituto de Fisiología Celular, and Alfonso Vilchis of Facultad de Ciencias, UNAM. We are grateful to L. Ongay and M. Mora (Unidad de Biología Molecular, Instituto de Fisiología Celular, UNAM) for the synthesis of deoxyoligonucleotides. Claudia Segal-Kischinevzky is grateful to the Posgrado en Ciencias Biológicas, UNAM, for the support during PhD studies. This work was supported partially by the Dirección General de Asuntos del Personal Académico, Universidad Nacional Autónoma de México (IN241602).

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Authors and Affiliations

  1. Biología Celular, Facultad de Ciencias, Universidad Nacional Autónoma de México, UNAM, Mexico, Mexico

    Claudia Segal-Kischinevzky, Beatriz Rodarte-Murguía, Victor Valdés-López & Luisa Alba-Lois

  2. Bioquímica, Facultad de Medicina, UNAM, Mexico, Mexico

    Guillermo Mendoza-Hernández

  3. Departamento de Bioquímica y Biología Estructural, Instituto de Fisiología Celular, UNAM, Mexico, Mexico

    Alicia González

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  1. Claudia Segal-Kischinevzky
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  6. Luisa Alba-Lois
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Correspondence to Luisa Alba-Lois.

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Segal-Kischinevzky, C., Rodarte-Murguía, B., Valdés-López, V. et al. The Euryhaline Yeast Debaryomyces hansenii has Two Catalase Genes Encoding Enzymes with Differential Activity Profile. Curr Microbiol 62, 933–943 (2011). https://doi.org/10.1007/s00284-010-9806-z

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  • DOI: https://doi.org/10.1007/s00284-010-9806-z

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