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Atypical Class 1 Integron Coexists with Class 1 and Class 2 Integrons in Multi-Drug Resistant Shigella flexneri Isolates from China

Current Microbiology volume 62, pages 802–806 (2011)Cite this article

Abstract

The antimicrobial resistance and the character of integrons were determined in 58 Shigella flexneri strains isolated from China. All isolates were multi-drug resistant and found to carry integrons of class 1 (94.8%), class 2 (100%), or both (94.8%). No intI3 was detected. The typical class 1 integrons were found in conjugative plasmids and could be transferred to the recipient E. coli DH5α. The gene cassettes of typical class 1 integrons dfrA17-aadA5 and dfrA12-orfF-aadA2 were detected in 54 strains (93.1%) and 1 strain, respectively. Atypical class 1 integrons located on the chromosome with gene cassettes bla oxa-30-aadA1 were detected in 55 isolates (94.8%). All the intI2 positive isolates carried gene cassettes dfrA1-sat1-aadA1. To our knowledge, this is the first report that atypical and typical class 1 integrons coexisted with class 2 integron in multi-drug resistant S. flexneri strains.

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Acknowledgments

This study was supported by the Research Fund from Ministry of Health of the People’s Republic China, WKJ2007-2-024.

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Affiliations

  1. Department of Environmental Health, College of Public Health, Zhengzhou University, Zhengzhou, People’s Republic of China

    Jing Yuan Zhu

  2. Department of Epidemiology, College of Public Health, Zhengzhou University, No. 100 Kexue Avenue, Zhengzhou, 450001, Henan, People’s Republic of China

    Guang Cai Duan, Hai Yan Yang & Yuan Lin Xi

  3. Henan University of Science & Technology, Luoyang, People’s Republic of China

    Guang Cai Duan

  4. Henan Key Laboratory of Molecular Medicine, Zhengzhou, People’s Republic of China

    Guang Cai Duan & Qing Tang Fan

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  1. Jing Yuan Zhu
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  2. Guang Cai Duan
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  3. Hai Yan Yang
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  4. Qing Tang Fan
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  5. Yuan Lin Xi
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Correspondence to Guang Cai Duan.

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Zhu, J.Y., Duan, G.C., Yang, H.Y. et al. Atypical Class 1 Integron Coexists with Class 1 and Class 2 Integrons in Multi-Drug Resistant Shigella flexneri Isolates from China. Curr Microbiol 62, 802–806 (2011). https://doi.org/10.1007/s00284-010-9790-3

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  • DOI: https://doi.org/10.1007/s00284-010-9790-3

Keywords

  • Restriction Fragment Length Polymorphism
  • Typical Class
  • Gene Cassette
  • Shigellosis
  • Shigella Flexneri