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Vadicella arenosi gen. nov., sp. nov., a Novel Member of the Class Alphaproteobacteria Isolated from Sandy Sediments from the Sea of Japan Seashore

Current Microbiology volume 62, pages 795–801 (2011)Cite this article

Abstract

A taxonomic study of three aerobic, Gram-negative, non-pigmented, non-motile rod-shaped bacterial strains, designated KMM 9008, KMM 9017, and KMM 9024T, which were isolated from a sandy sediment sample collected from the Sea of Japan seashore, was undertaken. The DNA–DNA hybridization values of 88–96% obtained between novel strains confirm their assignment to the same species. An analysis of the nearly complete 16S rRNA gene sequences showed that the novel isolates were closely related to each other (99.6–100% sequence similarity) and shared highest sequence similarities to the described genera Celeribacter (96.2–95.9%), Pseudoruegeria (95.6–94.3%), and Thalassobacter (95.2–93.1%) within the class Alphaproteobacteria. The major isoprenoid quinone was Q-10, polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidic acid, an unknown aminolipid and an unknown lipid as prevalent, and phosphatidylethanolamine was a minor component, and major fatty acids were C18:1 ω7c , followed by 11-Methyl C18:1 ω7c, C12:1 and C10:0 3-OH in all strains. The DNA G+C content of strains KMM 9008, KMM 9017, and KMM 9024T was in the range of 56.7–60 mol%. Based on distinctive phenotypic characteristics and phylogenetic distance, strain KMM 9024T (=NRIC 0787T = JCM 17190T) represents the type strain of a novel species in a novel genus, for which the name Vadicella arenosi gen. nov., sp. nov. is proposed.

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Acknowledgments

We would like to thank The Deutsche Sammlung von Microorganismen und Zellkulturen, DSMZ, Braunschweig, Germany, for providing strain Thalassobacter arenae DSM 19593T. This study was supported by a grant from the Presidium of RAS “Molecular and Cell Biology”, by a State Contract 02.518.11.7169 from the Federal Agency for Science and Innovations of the Russian Federation, and by a grant from Presidium Far-Eastern Branch of Russian Academy of Sciences 09-III-A-06_227.

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Affiliations

  1. Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, Russia, 690022

    Lyudmila A. Romanenko & Valery V. Mikhailov

  2. NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-Ku, Tokyo, 156-8502, Japan

    Naoto Tanaka

  3. Institute of Marine Biology, Far-Eastern Branch, Russian Academy of Sciences, Vladivostok, Russia, 690041

    Vasily I. Svetashev

Authors
  1. Lyudmila A. Romanenko
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  2. Naoto Tanaka
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  3. Vasily I. Svetashev
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  4. Valery V. Mikhailov
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Corresponding author

Correspondence to Lyudmila A. Romanenko.

Additional information

The DDBJ/GenBank/EMBL accession numbers of the 16S rRNA gene sequences of strains KMM 9008, KMM 9017, and KMM 9024T are AB564597, AB564596, and AB564595, respectively.

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Romanenko, L.A., Tanaka, N., Svetashev, V.I. et al. Vadicella arenosi gen. nov., sp. nov., a Novel Member of the Class Alphaproteobacteria Isolated from Sandy Sediments from the Sea of Japan Seashore. Curr Microbiol 62, 795–801 (2011). https://doi.org/10.1007/s00284-010-9780-5

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  • DOI: https://doi.org/10.1007/s00284-010-9780-5

Keywords

  • Alphaproteobacteria
  • Itaconic Acid
  • Marine 2216 Agar
  • Marine Broth
  • Isoprenoid Quinone