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Table 1 Bacterial strains and plasmids used in this work

From: Assessment of the Mobilizable Vector Plasmids pSUP202 and pSUP404.2 as Genetic Tools for the Predatory Bacterium Bdellovibrio bacteriovorus

  Description Source
Strains
 B. bacteriovorus HD100-S1 Streptomycin resistant wild-type of HD100 (DSM50701) prey-dependent BfR strain collectiona
 B. bacteriovorus M1 Saprophytic HD100 mutant strain This study
 B. bacteriovorus M1.1 Axenic HD100 mutant strain This study
 E. coli K-12   DSM423
 E. coli JC3272 K-12, streptomycin resistant [7]
 E. coli K-12 S17.1λpir RecA thio, hsdRM, Integrated plasmid RP4-2-Kn::Tn7/TC::M DSM9526
 E. coli K-12 Gene Hogs® F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2 Invitrogen, Darmstadt, Germany
Plasmids
 pSUP202 pMB1 replicon, ApR, TcR, CMR, mob [15]
 pSUP404.2 p15A replicon, RSF1010 replicon, KnR, CmR, mob [9]
 pGFPuv pMB1 replicon, ApR, gfpuv Clontech (Mountain View, USA)
 pBR329 pMB1 replicon; ApR, TcR, CmR [3]
 pIV2 p29807 replicon, KnR [17]
 pNR24 Insertion of Plac and gfp uv into pSUP404.2 via the restriction sites NheI and HindIII This study
  1. aSpontaneous mutant isolated by Dominik Schwudke (pers. communication)