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Current Microbiology

, Volume 62, Issue 2, pp 532–538 | Cite as

Toxicological Effects of Selective Herbicides on Plant Growth Promoting Activities of Phosphate Solubilizing Klebsiella sp. Strain PS19

  • Munees Ahemad
  • Md. Saghir KhanEmail author
Article

Abstract

This study examines the effect of four herbicides, quizalafop-p-ethyl, clodinafop, metribuzin and glyphosate, on plant growth promoting activities like phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production by herbicide tolerant Klebsiella sp. strain PS19. The strain was isolated from mustard rhizosphere. The selected herbicides were applied two to three times at the recommended rates. Klebsiella sp. strain PS19 tolerated a concentration of 1600 μg/ml each of quizalafop-p-ethyl and clodinafop, and 3200 and 2800 μg/ml of metribuzin and glyphosate, respectively. The activities of Klebsiella sp. strain PS19 observed under in vitro environment were persistent in the presence of all herbicides at lower rates. The plant growth promoting activities even-though decreased regularly, but was not lost completely, as the concentration of each herbicide was increased from the recommended to three times of higher doses. Among all herbicides, quizalafop-p-ethyl, generally, showed maximum toxicity to plant growth promoting activities of Klebsiella sp. strain PS19. As an example, 40, 80 and 120 μg/l of quizalafop-p-ethyl added to liquid culture Pikovskaya medium, decreased phosphate solubilizing activity of strain PS19 by 93, 95 and 97%, respectively over untreated control. The study revealed that the higher rates of herbicides though decreased the plant growth promoting activity but it did not completely inhibit the metabolic activities of strain PS19. The herbicide tolerance together with growth promoting activities observed under herbicide stress suggests that Klebsiella sp. strain PS19 could be used as bacterial preparation for facilitating the growth and yields of crops even in soils polluted with herbicides.

Keywords

Glyphosate Indole Acetic Acid Plant Growth Promote Indole Acetic Acid Plant Growth Promote Rhizobacteria 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Ahemad M, Khan MS (2010) Phosphate-solubilizing and plant-growth-promoting Pseudomonas aeruginosa PS1 improves greengram performance in quizalafop-p-ethyl and clodinafop amended soil. Arch Environ Contam Toxicol 58:361–372CrossRefPubMedGoogle Scholar
  2. 2.
    Ahemad M, Khan MS, Zaidi A, Wani PA (2009) Remediation of herbicides contaminated soil using microbes. In: Khan MS, Zaidi A, Musarrat J (eds) Microbes in sustainable agriculture. Nova Publishers, New YorkGoogle Scholar
  3. 3.
    Abbas-Zadeh P, Saleh-Rastin N, Asadi-Rahmani H, Khavazi K, Soltani A, Shoary-Nejati AR, Miransari M (2010) Plant growth-promoting activities of fluorescent pseudomonads, isolated from the Iranian soils. Acta Physiol Plant 32:281–288CrossRefGoogle Scholar
  4. 4.
    Alexander DB, Zuberer DA (1991) Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 12:39–45CrossRefGoogle Scholar
  5. 5.
    Babalola OO (2010) Beneficial bacteria of agricultural importance. Biotechnol Lett. doi:  10.1007/s10529-010-0347-0
  6. 6.
    Babita S, Sunita S, Kamlesh K (2009) Impact of long term use of clodinafop in wheat on soil microbes. Ind J Weed Sci 41:50–53Google Scholar
  7. 7.
    Bakker AW, Schipper B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp. mediated plant growth stimulation. Soil Biol Biochem 19:451–457CrossRefGoogle Scholar
  8. 8.
    Bellinaso ML, Greer CW, Peralba MC, Henriques JA, Gaylarde CC (2003) Biodegradation of the herbicide trifluralin by bacteria isolated from soil. FEMS Microbiol Ecol 43:191–194CrossRefGoogle Scholar
  9. 9.
    Boldt TS, Jacobsen CS (1998) Different toxic effects of the sulphonylurea herbicides metsulfuron methyl, chlorsulfuron and thifensulfuron methyl on fluorescent pseudomonads isolated from an agricultural soil. FEMS Microbiol Lett 161:29–35CrossRefGoogle Scholar
  10. 10.
    Brick JM, Bostock RM, Silversone SE (1991) Rapid In situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl Environ Microbiol 57:535–538Google Scholar
  11. 11.
    Chen YP, Rekha PD, Arun AB, Shen FT, Lai WA, Young CC (2006) Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol 34:33–41CrossRefGoogle Scholar
  12. 12.
    Chitra RS, Sumitra VC, Yash DS (2002) Effect of different nitrogen sources and plant growth regulators on glutamine synthetase and glutamate synthase activities of radish cotyledons. Bulg J Plant Physiol 28:46–56Google Scholar
  13. 13.
    Chung H, Park M, Madhaiyan M, Seshadri S, Song J, Cho H, Sa T (2005) Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea. Soil Biol Biochem 37:1970–1974CrossRefGoogle Scholar
  14. 14.
    Das AC, Debnath A, Mukherjee D (2003) Effect of the herbicides oxadiazon and oxyfluorfen on phosphates solubilizing microorganisms and their persistence in rice fields. Chemosphere 53:217–221CrossRefPubMedGoogle Scholar
  15. 15.
    Devi KK, Seth N, Kothamasi S, Kothamasi D (2007) Hydrogen cyanide-producing rhizobacteria kill subterranean termite Odontotermes obesus rambur. by cyanide poisoning under in vitro conditions. Curr Microbiol 54:74–78CrossRefPubMedGoogle Scholar
  16. 16.
    Dye DW (1962) The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. NZJ Sci 5:393–416Google Scholar
  17. 17.
    Erturk Y, Ercisli S, Haznedar A, Cakmakci R (2010) Effects of plant growth promoting rhizobacteria (PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings. Biol Res 43:91–98CrossRefPubMedGoogle Scholar
  18. 18.
    Gordon S, Weber RP (1951) The calorimetric estimation of IAA. Plant Physiol 26:192–195CrossRefPubMedGoogle Scholar
  19. 19.
    Herman PL, Behrens M, Chakraborty S, Crastil BM, Barycki J, Weeks DP (2005) A three component dicamba O-demethylase from Pseudomonas maltiphilia strain DI-6: gene isolation, characterization and heterologous expression. J Biol Chem 280:24759–24767CrossRefPubMedGoogle Scholar
  20. 20.
    Holt JG, Krieg NR, Sneath PHA, Staley JT, Willams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, BaltimoreGoogle Scholar
  21. 21.
    Indiragandhi P, Anandham R, Madhaiyan M, Sa TM (2008) Characterization of plant growth-promoting traits of bacteria isolated from larval guts of diamondback moth Plutella xylostella Lepidoptera: Plutellidae. Curr Microbiol 56:327–333CrossRefPubMedGoogle Scholar
  22. 22.
    Islam MR, Madhaiyan M, Deka Boruah HP, Yim W, Lee G, Saravanan VS, Fu Q, Hu H, Sa T (2009) Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants. J Microbiol Biotechnol 19:1213–1222CrossRefPubMedGoogle Scholar
  23. 23.
    Jackson ML (1967) Soil chemical analysis, 1st edn. Prentice Hall of India Pvt. Ltd, New DelhiGoogle Scholar
  24. 24.
    Johnsen K, Jacobsen CS, Torsvik V, Sorensen J (2001) Pesticide effects on bacterial diversity in agricultural soils-a review. Biol Fertil Soils 33:443–453CrossRefGoogle Scholar
  25. 25.
    Kapoor K, Leenta Arora (1996) Observations on growth responses of cyanobacteria under the influence of herbicides. Pollut Res 15:343–351Google Scholar
  26. 26.
    Khan MS, Zaidi A, Ahemad M, Oves M, Wani PA (2010) Plant growth promotion by phosphate solubilizing fungi—current perspective. Arch Agron Soil Sci 56:73–98CrossRefGoogle Scholar
  27. 27.
    King JE (1932) The colorimetric determination of phosphorus. Biochem J 26:292–297PubMedGoogle Scholar
  28. 28.
    Kumar S, Mukerji KG, Lal R (1996) Molecular aspects of pesticide degradation by microorganisms. Crit Rev Microbiol 22:1–26CrossRefPubMedGoogle Scholar
  29. 29.
    Lugtenberg B, Kamilova F (2009) Plant-growth-promoting rhizobacteria. Annu Rev Microbiol 63:541–556CrossRefPubMedGoogle Scholar
  30. 30.
    Madhaiyan M, Poonguzhali S, Hari K, Saravanan VS, Sa T (2006) Influence of pesticides on the growth rate and plant-growth promoting traits of Gluconacetobacter diazotrophicus. Pestic Biochem Physiol 84:143–154CrossRefGoogle Scholar
  31. 31.
    Mody BR, Bindra MO, Modi VV (1989) Extracellular polysaccharides of cowpea rhizobia: compositional and functional studies. Arch Microbiol 153:38–42CrossRefGoogle Scholar
  32. 32.
    Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology 17:362–370Google Scholar
  33. 33.
    Poonguzhali S, Madhaiyan M, Sa T (2008) Isolation and identification of phosphate solubilizing bacteria from chinese cabbage and their effect on growth and phosphorus utilization of plants. J Microbiol Biotechnol 18:773–777PubMedGoogle Scholar
  34. 34.
    Reeves MW, Pine L, Neilands JB, Balows A (1983) Absence of siderophore activity in Legionella species grown in iron-deficient media. J Bacteriol 154:324–329PubMedGoogle Scholar
  35. 35.
    Sachdev DP, Chaudhari HG, Kasture VM, Dhavale DP, Chopade BA (2009) Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian J Exp Biol 47:993–1000PubMedGoogle Scholar
  36. 36.
    Sándor Z, Kátai J, Tállai M, Varga A, Balogh E (2007) The effect of herbicides applied in maize on the dynamics of some soil microbial groups and soil enzyme activity. Cereal Res Commun 35:1025–1028CrossRefGoogle Scholar
  37. 37.
    Sarode PD, Rane MR, Chaudhari BL, Chincholkar SB (2009) Siderophoregenic Acinetobacter calcoaceticus isolated from wheat rhizosphere with strong PGPR activity. Malaysian J Microbiol 5:6–12Google Scholar
  38. 38.
    Srinivas T, Sridevi M, Mallaiah KV, India G, Nagar N (2008) Effect of pesticides on Rhizobium and nodulation of green gram Vigna radita (L.) Wilczek. ICFAI J Life Sci 2:36–44Google Scholar
  39. 39.
    Tank N, Saraf M (2003) Phosphate solubilization, exopolysaccharide production and indole acetic acid secretion by rhizobacteria isolated from Trigonella foenum-graecum. Indian J Microbiol 43:37–40Google Scholar
  40. 40.
    Zaidi A, Khan MS, Ahemad M, Oves M (2009) Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiol Immunol Hung 56:263–284CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Agricultural Microbiology, Faculty of Agricultural SciencesAligarh Muslim UniversityAligarhIndia

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