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The Novel Responses of Ethambutol Against Mycobacterium smegmatis mc2155 Revealed by Proteomics Analysis

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Abstract

Ethambutol (EMB), one of the effective anti-mycobacterial drugs, inhibits the biosynthesis of mycobacterium cell wall. To elucidate the molecular mechanism of EMB against tuberculosis (TB), Mycobacterium smegmatis mc2155 was employed as a model of mycobacterial system in this study. We compared the protein profiles on M. smegmatis mc2155 treated by EMB and untreated using fluorescence difference two-dimensional gel electrophoresis (2-D DIGE). A total of 40 differential protein spots were selected and 22 proteins were identified by HPLC-nano ESI–MS/MS analysis, including 16 over-expressed proteins and 6 under-expressed proteins. These proteins mainly affected energy metabolism, as well as synthesis and modification of macromolecules. The expressions of correspondent genes were confirmed by RT-PCR. This investigation provided some clues for searching potential drug targets.

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Acknowledgments

This study is supported by the grants from National Natural Science Foundation of China (30970647), National Basic Research Program of China (2006CB504400) and Key Project of Major Infectious Diseases (2008ZX10003-006).

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Authors and Affiliations

  1. Department of Biotechnology, Dalian Medical University, Dalian, 116044, China

    Tao Jiang, Mingzhong Sun, Shizhu Zang & Yi Xin

  2. Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian, 116044, China

    Yaoyao Zhan, Shuqing Liu & Yufang Ma

  3. The Institute of Life Science and Technology, Dalian Ocean University, Dalian, 116023, China

    Yaoyao Zhan

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  1. Tao Jiang
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  2. Yaoyao Zhan
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  3. Mingzhong Sun
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  4. Shuqing Liu
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  5. Shizhu Zang
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  6. Yufang Ma
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  7. Yi Xin
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Correspondence to Yi Xin.

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Jiang, T., Zhan, Y., Sun, M. et al. The Novel Responses of Ethambutol Against Mycobacterium smegmatis mc2155 Revealed by Proteomics Analysis. Curr Microbiol 62, 341–345 (2011). https://doi.org/10.1007/s00284-010-9711-5

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  • DOI: https://doi.org/10.1007/s00284-010-9711-5

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