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Luteibacter jiangsuensis sp. nov.: A Methamidophos-Degrading Bacterium Isolated from a Methamidophos-Manufacturing Factory

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Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, JW-64-1T, capable of degrading methamidophos was isolated from a methamidophos-manufacturing factory in China, and was subjected to a polyphasic taxonomic investigation. Strain JW-64-1T produced circular, smooth, transparent, yellow-colored colonies (1.0–2.0 mm) on LB agar after 2 days incubation. It grew optimally at 25–30°C and pH 7.0 without the presence of NaCl. The G+C content of the total DNA was 63.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain JW-64-1T fell within the cluster comprising Luteibacter species. The 16S rRNA gene sequence of strain JW-64-1T was most closely related to Luteibacter rhizovicinus DSM 16549T (98.6%), followed by Luteibacter yeojuensis DSM 17673T (98.4%) and L. anthropi CCUG 25036T (98.2%). The major cellular fatty acids of strain JW-64-1T were iso-C15:0 (24.1%), iso-C17:0 (20.2%) and summed feature 9 comprising iso-C17:1 ω9c and/or C16:0 10-methyl (20.3%). The major isoprenoid quinine was Q-8 (98%), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphoaminolipid, aminolipids-1, aminolipids-2, and phospholipids. The values for DNA–DNA relatedness between strain JW-64-1T and the closest phylogenetic relatives of L. rhizovicinus and Luteibacter yeojuensis were 34.8 ± 2.6 and 25.6 ± 3.1%, respectively. On the basis of the phenotypic, chemotaxonomic, DNA–DNA relatedness and phylogenetic analysis based on the 16S rRNA gene sequences, strain JW-64-1T represents a novel species of the genus Luteibacter, for which the name Luteibacter jiangsuensis sp. nov. is proposed. The type strain is JW-64-1T (=CGMCC 1.10133T = DSM 22396T).

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Acknowledgments

We are grateful to Dr. B. J. Tindall of DSMZ, Braunschweig, Germany, for the analysis of respiratory quinones and polar lipids. This work was supported by grants from the National Programs for High Technology Research and Development of China (2007AA10Z405), the Major Projects on Control and Rectification of Water Body Pollution (2009ZX07103-002, 2008ZX07316-002) and Science & Technology Program of Ministry of Construction of People’s Republic of China (2009-K4-3).

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Authors and Affiliations

  1. Department of Microbiology, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People’s Republic of China

    Li Wang, Guang-li Wang, Shun-peng Li & Jian-dong Jiang

  2. Department of Environment and Enengy Engineering, Anhui University of Architecture, Hefei, 230022, People’s Republic of China

    Li Wang

  3. College of Life Sciences, Huaibei Normal University, Huaibei, 235000, People’s Republic of China

    Guang-li Wang

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  1. Li Wang
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  2. Guang-li Wang
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  3. Shun-peng Li
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  4. Jian-dong Jiang
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Corresponding author

Correspondence to Jian-dong Jiang.

Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JW-64-1T is FJ848571.

L. Wang and G. Wang contributed equally to this work.

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Wang, L., Wang, Gl., Li, Sp. et al. Luteibacter jiangsuensis sp. nov.: A Methamidophos-Degrading Bacterium Isolated from a Methamidophos-Manufacturing Factory. Curr Microbiol 62, 289–295 (2011). https://doi.org/10.1007/s00284-010-9707-1

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