Current Microbiology

, Volume 62, Issue 1, pp 182–190 | Cite as

The Diversity and Anti-Microbial Activity of Endophytic Actinomycetes Isolated from Medicinal Plants in Panxi Plateau, China

  • Ke Zhao
  • Petri Penttinen
  • Tongwei Guan
  • Jing Xiao
  • Qiang Chen
  • Jun Xu
  • Kristina Lindström
  • Lili Zhang
  • Xiaoping Zhang
  • Gary A. Strobel
Article

Abstract

Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in South-west Sichuan in China with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. In this study, 560 endophytic actinomycetes were isolated from 26 medicinal plant species in Panxi plateau. 60 isolates were selected for 16S rDNA-RFLP analysis and 14 representative strains were chosen for 16S rDNA sequencing. According to the phylogenetic analysis, seven isolates were Streptomyces sp., while the remainder belonged to genera Micromonospora, Oerskovia, Nonomuraea, Promicromonospora and Rhodococcus. Antimicrobial activity analysis combined with the results of amplifying genes coding for polyketide synthetase (PKS-I, PKS-II) and nonribosomal peptide synthetase (NRPS) showed that endophytic actinomycetes isolated from medicinal plants in Panxi plateau had broad-spectrum antimicrobial activity and potential natural product diversity, which further proved that endophytic actinomycetes are valuable reservoirs of novel bioactive compounds.

Supplementary material

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Supplementary material 1 (DOC 136 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Ke Zhao
    • 1
  • Petri Penttinen
    • 2
  • Tongwei Guan
    • 1
    • 3
  • Jing Xiao
    • 4
  • Qiang Chen
    • 1
  • Jun Xu
    • 4
  • Kristina Lindström
    • 2
  • Lili Zhang
    • 3
  • Xiaoping Zhang
    • 1
  • Gary A. Strobel
    • 5
  1. 1.Department of Microbiology, College of Resource and Environmental SciencesSichuan Agricultural UniversityYaanPeople’s Republic of China
  2. 2.Department of Applied Chemistry and MicrobiologyUniversity of HelsinkiHelsinkiFinland
  3. 3.Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production & Construction CorpsTarim UniversityAlarPeople’s Republic of China
  4. 4.Key Laboratory of Marine Biogenetic Resources, The Third Institute of OceanographyState Oceanic AdministrationXiamenPeople’s Republic of China
  5. 5.Department of Plant SciencesMontana State UniversityBozemanUSA

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