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Current Microbiology

, Volume 62, Issue 1, pp 133–138 | Cite as

Expression of Divergent LSU rRNA Genes in the Vibrio vulnificus CMCP6 Genome During Both Infection and Non-Pathogenic Stages

  • Hyun-Lee Kim
  • Sang-Mi Ryou
  • Minho Lee
  • Jin-Won Lee
  • Kangseok LeeEmail author
  • Jeehyeon BaeEmail author
Article

Abstract

The Vibrio vulnificus CMCP6 genome harbors nine copies of divergent large subunit (LSU) rRNA genes that may express and constitute four kinds of LSU rRNA molecules in a single cell. Primer extension analyses showed that these heterogeneous LSU rRNA transcripts are all expressed and assembled into ribosomes during both infection and nonpathogenic stages. Phylogenetic analyses of the internal transcribed spacer between SSU and LSU genes indicated that rRNA operons of V. vulnificus CMCP6 can be clustered into three distinct groups in rRNA genes of closely related Vibrio species. These findings imply that divergent rRNA genes in V. vulnificus CMCP6 resulted from interspecies recombination events in V. species, while the consequences of expression of heterogeneous rRNA molecules are not clear.

Keywords

Internal Transcribe Spacer Vibrio Vibrio Species rRNA Operon Primer Extension Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by National Research Foundation of Korea Grant funded by the Korean Government (2009-0073671) and Seoul R&BD program (10543).

Supplementary material

284_2010_9684_MOESM1_ESM.pdf (1.9 mb)
Supplementary material 1 (PDF 1926 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Biomedical Science, College of Life ScienceCHA UniversitySeongnamRepublic of Korea
  2. 2.Department of Life ScienceChung-Ang UniversitySeoulRepublic of Korea
  3. 3.Department of Life ScienceHanyang UniversitySeoulRepublic of Korea

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