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Current Microbiology

, Volume 62, Issue 1, pp 126–132 | Cite as

Characterization of the Antiviral Activity for Influenza Viruses M1 Zinc Finger Peptides

  • Yongjin Wang
  • Huihui Xiao
  • Nannan Wu
  • Huiling Shi
  • Hongwei Xu
  • Lichen Zhou
  • Xu-Guang Xi
  • Tianhou Wang
  • Xiaoming WangEmail author
Article

Abstract

We sought to investigate the cellular uptake and antiviral activity for the M1 zinc finger peptides derived from influenza A and influenza B viruses in vitro. No cellular uptake was detected by fluorescent microscopy for the synthetic zinc finger peptides. When flanked to a cell permeable peptide Tp10, the zinc finger recombinant proteins were efficiently internalized by MDCK cells. However, no antiviral activity was detected against homologous or heterologous virus infections for the synthetic peptides or the Tp10-flanked recombinant proteins, regardless treated with or without Zn2+. Nevertheless, MDCK cell constitutively expressing the M1 zinc finger peptides in cell nuclei potently inhibited replication of homologous, but not heterologous influenza viruses. Adenoviral vector delivered M1 zinc finger peptides also exhibited potent antiviral activity against homologous viruses challenge. Transduction at 100 PFU dose of recombinant adenovirus efficiently protected 99% of the cells from 100 TCID50 of different virus infections for both peptides. These results brought new insight to the antiviral researches against influenza virus infections.

Keywords

Influenza Antiviral Activity Synthetic Peptide MDCK Cell Adenoviral Vector 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by grants from Shanghai Municipal Science and Technology Commission (No. 07DZ22940) and Shanghai Municipal Wildlife Administration (No. SBHZ2006_01).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Yongjin Wang
    • 1
  • Huihui Xiao
    • 1
  • Nannan Wu
    • 1
  • Huiling Shi
    • 1
  • Hongwei Xu
    • 1
  • Lichen Zhou
    • 1
  • Xu-Guang Xi
    • 2
  • Tianhou Wang
    • 1
  • Xiaoming Wang
    • 1
    Email author
  1. 1.Laboratory of Wildlife Epidemic DiseasesEast China Normal UniversityShanghaiChina
  2. 2.CNRS, UMR 2027, Institut Curie-Section de RechercheCentre UniversitaireOrsayFrance

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