Characterization of the Antiviral Activity for Influenza Viruses M1 Zinc Finger Peptides
- 153 Downloads
We sought to investigate the cellular uptake and antiviral activity for the M1 zinc finger peptides derived from influenza A and influenza B viruses in vitro. No cellular uptake was detected by fluorescent microscopy for the synthetic zinc finger peptides. When flanked to a cell permeable peptide Tp10, the zinc finger recombinant proteins were efficiently internalized by MDCK cells. However, no antiviral activity was detected against homologous or heterologous virus infections for the synthetic peptides or the Tp10-flanked recombinant proteins, regardless treated with or without Zn2+. Nevertheless, MDCK cell constitutively expressing the M1 zinc finger peptides in cell nuclei potently inhibited replication of homologous, but not heterologous influenza viruses. Adenoviral vector delivered M1 zinc finger peptides also exhibited potent antiviral activity against homologous viruses challenge. Transduction at 100 PFU dose of recombinant adenovirus efficiently protected 99% of the cells from 100 TCID50 of different virus infections for both peptides. These results brought new insight to the antiviral researches against influenza virus infections.
KeywordsInfluenza Antiviral Activity Synthetic Peptide MDCK Cell Adenoviral Vector
This study was supported by grants from Shanghai Municipal Science and Technology Commission (No. 07DZ22940) and Shanghai Municipal Wildlife Administration (No. SBHZ2006_01).
- 4.Gozdek A, Zhukov I, Polkowska A, Poznanski J, Stankiewicz-Drogon A, Pawlowicz JM, Zagorski-Ostoja W, Borowski P, Boguszewska-Chachulska AM (2008) NS3 Peptide, a novel potent hepatitis C virus NS3 helicase inhibitor: its mechanism of action and antiviral activity in the replicon system. Antimicrob Agents Ch 52:393–401CrossRefGoogle Scholar
- 8.Judd AK, Sanchez A, Bucher DJ, Huffman JH, Bailey K, Robert W, Sidwell RW (1997) In vivo anti-influenza virus activity of a zinc finger peptide. Antimicrob Agents Ch 41:687–692Google Scholar