The regulatory function of extracellular ATP (exATP) in bacteria is unknown, but recent studies have demonstrated exATP induced enhanced secondary metabolite production and morphological differentiation in Streptomyces coelicolor. The growth of Streptomyces coelicolor, however, was unaffected by exATP, although changes in growth are common phenotypes. To identify bacteria whose growth is altered by exATP, we measured exATP-induced population changes in fast-growing microbes and actinomycetes in compost. Compared with the water-treated control, the addition of 10 ml 100 μM ATP to 10 g of compost enhanced the actinomycetes population by 30% and decreased fast-growing microbial numbers by 20%. Eight microbes from each group were selected from the most populated colony, based on appearance. Of the eight isolated fast-growing microbes, the 16S rRNA sequences of three isolates were similar to the plant pathogens Serratia proteamaculans and Sphingomonas melonis, and one was close to a human pathogen, Elizabethkingia meningoseptica. The growth of all fast-growing microbes was inhibited by ATP, which was confirmed in Pseudomonassyringae DC3000, a pathogenic plant bacterium. The growth of six of eight isolated actinomycetes strains, all of which were identified as close to Streptomyces neyagawaensis, was enhanced by ATP treatment. This study suggests that exATP regulates bacterial physiology and that the exATP response system is a target for the control of bacterial ecology.
Streptomyces Secondary Metabolite Production Bacterial Physiology Populated Colony Bacterial Ecology
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This work was supported by a grant (11-2008-16-001-00) from the 21C Frontier Microbial Genomics and Application Center program, the Korean Ministry of Science and Technology and by a National Research Foundation of Korea (NRF) grant, funded by the Korea government (MEST) (#359-208-1-F00002). Sung-Kwon Lee supported by the second stage of BK21 (Brain Korea 21) Project.
Bentley SD, Chater KF, Cerdeno-Tarraga AM et al (2002) Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141–147CrossRefPubMedGoogle Scholar
Carrero-Colon M, Nakatsu CH, Konopka A (2006) Effect of nutrient periodicity on microbial community dynamics. Appl Environ Microb 72:3175–3183CrossRefGoogle Scholar
Chamberlain K, Crawford DL (1999) In vitro and in vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopicus strains YCED9 and WYE53. J Ind Microbiol Biotechnol 23:641–646CrossRefPubMedGoogle Scholar
Chun J, Goodfellow M (1995) A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45:240–245CrossRefPubMedGoogle Scholar
Doumbou C-L, Akimov V, Cote M et al (2001) Taxonomic study on nonpathogenic streptomycetes isolated from common scab lesions on potato tubers. Syst Appl Microbiol 24:451–456CrossRefPubMedGoogle Scholar
Dufour D, Nicodème M, Perrin C et al (2008) Molecular typing of industrial strains of Pseudomonas spp. isolated from milk and genetical and biochemical characterization of an extracellular protease produced by one of them. Int J Food Microbiol 125:188–196CrossRefPubMedGoogle Scholar
Gneiding K, Frodl R, Funke G (2008) Identities of Microbacterium spp. encountered in human clinical specimens. J Clin Microbiol 46:3646–3652CrossRefPubMedGoogle Scholar
Grkovic S, Glare TR, Jackson TA et al (1995) Genes essential for amber disease in grass grubs are located on the large plasmid found in Serratia entomophila and Serratia proteamaculans. Appl Environ Microb 61:2218–2223Google Scholar
Gulati A, Rahi P, Vyas P (2008) Characterization of phosphate-solubilizing fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas. Curr Microbiol 56:73–79CrossRefPubMedGoogle Scholar
Hayakawa M, Nonomura H (1987) Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65:501–509CrossRefGoogle Scholar
Haydock SF, Appleyard AN, Mironenko T et al (2005) Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449. Microbiology 151:3161–3169CrossRefPubMedGoogle Scholar
Hopwood DA, Kieser T, Wright HM et al (1983) Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol 129:2257–2269PubMedGoogle Scholar
Huang Y-J, Maruyama Y, Dvoryanchikov G et al (2007) The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds. Proc Natl Acad Sci USA 104:6436–6441CrossRefPubMedGoogle Scholar
Jeter CR, Tang W, Henaff E et al (2004) Evidence of a novel cell signaling role for extracellular adenosine triphosphates and diphosphates in Arabidopsis. Plant Cell 16:2652–2664CrossRefPubMedGoogle Scholar
Kim KK, Kim MK, Lim JH et al (2005) Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningosepticacomb. nov. and Elizabethkingia miricolacomb. nov. Int J Syst Evol Microbiol 55:1287–1293CrossRefPubMedGoogle Scholar
Kim S-Y, Sivaguru M, Stacey G (2006) Extracellular ATP in plants. Visualization, localization, and analysis of physiological significance in growth and signaling. Plant Physiol 142:984–992CrossRefPubMedGoogle Scholar
Kim S-H, Yang SH, Kim T-J et al (2009) Hypertonic stress increased extracellular ATP levels and the expression of stress-responsive genes in Arabidopsis thaliana seedlings. Biosci Biotechnol Biochem 73:1252–1256CrossRefPubMedGoogle Scholar
Krechel A, Faupel A, Hallmann J et al (2002) Potato-associated bacteria and their antagonistic potential towards plant-pathogenic fungi and the plant-parasitic nematode Meloidogyne incognita (Kofoid & White) Chitwood. Can J Microbiol 48:772–786CrossRefPubMedGoogle Scholar
Kuster E, Williams ST (1964) Selection of media for isolation of streptomycetes. Nature 202:928–929CrossRefGoogle Scholar
Lambert B, Meire P, Joos H et al (1990) Fast-growing, aerobic, heterotrophic bacteria from the rhizosphere of young sugar beet plants. Appl Environ Microb 56:3375–3381Google Scholar
Lee JS, Kook SY, Yoon JH et al (2001) Sphingomonas aquatilis sp. nov., Sphingomonas koreensis sp. nov. and Sphingomonas taejonensis sp. nov., yellow-pigmented bacteria isolated from natural mineral water. Int J Syst Bacteriol 51:1491–1498Google Scholar
Li M, Kim T-J, Kwon H-J et al (2008) Effects of extracellular ATP on the physiology of Streptomyces coelicolor A3(2). FEMS Microbiol Lett 286:24–31CrossRefPubMedGoogle Scholar
Ortega-Morales BO, Santiago-Garc JL, Chan-Bacab MJ et al (2007) Characterization of extracellular polymers synthesized by tropical intertidal biofilm bacteria. J Appl Microbiol 102:254–264CrossRefPubMedGoogle Scholar
Samac DA, Kinkel LL (2001) Suppression of the root-lesion nematode (Pratylenchus penetrans) in alfalfa (Medicago sativa) by Streptomyces spp. Plant Soil 235:35–44CrossRefGoogle Scholar
Sproer C, Mendrock U, Swiderski J et al (1999) The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae. Int J Syst Bacteriol 49:1433–1438CrossRefPubMedGoogle Scholar
Thomas C, Sun Y, Naus K et al (1999) Apyrase functions in plant phosphate nutrition and mobilizes phosphate from extracellular ATP. Plant Physiol 119:543–552CrossRefPubMedGoogle Scholar
Zhang X, Chen Y, Wang C et al (2007) Neuronal somatic ATP release triggers neuron-satellite glial cell communication in dorsal root ganglia. Proc Natl Acad Sci USA 104:9864–9869CrossRefPubMedGoogle Scholar