Advertisement

Current Microbiology

, Volume 62, Issue 1, pp 64–70 | Cite as

Effects of Age and Region on Fecal Microflora in Elderly Subjects Living in Bama, Guangxi, China

  • Liang Zhao
  • Wentao Xu
  • Salam A. Ibrahim
  • Junhua Jin
  • Jiannan Feng
  • Jingli Jiang
  • Jianjun Meng
  • Fazheng RenEmail author
Article

Abstract

Intestinal microflora analysis was performed on 52 healthy elderly subjects of different ages and in different regions in Bama County, Guangxi, China. The participants were assigned to three groups depending on their age and location: longevous (group M; mean age = 98 years; n = 21); rural younger elderly (group S; mean age = 70 years; n = 18); and urban elderly (group C; mean age = 82 years; n = 13). Ten groups of bacteria were quantified using real-time PCR. Age-related differences were observed in the number of Clostridium coccoidesEubacterium rectale—there were more in longevous participants. Region affected the numbers of BacteroidesPrevotella and Clostridium perfringens subgroup, and longevous participants had significantly more of the two bacterial groups than urban elderly participants. Region-related effects were also observed for the relative abundance of E. coli, and rural elderly participants had a lower proportion. Both age and regional effects were observed in the amount of total bacteria, and longevous participants had higher numbers than urban elderly participants. A significantly higher proportion of lactobacilli was observed in rural younger elderly participants than urban elderly participants, but independent age or regional effects did not contribute to this difference. This study suggests that age and region can affect the intestinal microflora of elderly people.

Keywords

Lactobacillus Clostridium Bacterial Group Genome Copy Total Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was funded by the Ministry of Science and Technology of China (2006BAD04A06 and 2007AA10Z311). The authors thank Rui Qin (Bureau of Science and Technology of Bama Yao Autonomous County) for his assistance in volunteer recruitment and sample collection.

References

  1. 1.
    Bartosch S, Fite A, Macfarlane GT, McMurdo MET (2004) Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl Environ Microbiol 70:3575–3581CrossRefPubMedGoogle Scholar
  2. 2.
    Fogel GB, Collins CR, Li J, Brunk CF (1999) Prokaryotic genome size and SSU rDNA copy number: estimation of microbial relative abundance from a mixed population. Microb Ecol 38:93–113CrossRefPubMedGoogle Scholar
  3. 3.
    Gibson GR, Roberfroid MB (1995) Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 125:1401–1412PubMedGoogle Scholar
  4. 4.
    Hayashi H, Sakamoto M, Kitahara M, Benno Y (2003) Molecular analysis of fecal microbiota in elderly individuals using 16S rDNA library and T-RFLP. Microbiol Immunol 47:557–570PubMedGoogle Scholar
  5. 5.
    He T, Harmsen HJM, Raangs GC, Welling GW (2003) Composition of faecal microbiota of elderly people. Microb Ecol Health Dis 15:153–159CrossRefGoogle Scholar
  6. 6.
    Hébuterne X (2003) Gut changes attributed to ageing: effects on intestinal microflora. Curr Opin Clin Nutr Metab Care 6:49–54CrossRefPubMedGoogle Scholar
  7. 7.
    Heilig HGHJ, Zoetendal EG, Vaughan EE, Marteau P, Akkermans ADL, De Vos WM (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68:114–123CrossRefPubMedGoogle Scholar
  8. 8.
    Hopkins MJ, Macfarlane GT (2002) Changes in predominant bacterial populations in human faeces with age and with Clostridium difficile infection. J Med Microbiol 51:448–454PubMedGoogle Scholar
  9. 9.
    Hopkins MJ, Sharp R, Macfarlane GT (2001) Age and disease related changes in intestinal bacterial populations assessed by cell culture, 16S rRNA abundance, and community cellular fatty acid profiles. Gut 48:198–205CrossRefPubMedGoogle Scholar
  10. 10.
    Komai M, Nanno M (1992) Intestinal microflora and longevity. In: Nakazawa Y, Hosono A (eds) Functions of fermented milk. Elsevier Applied Science, London, UK, pp 325–353Google Scholar
  11. 11.
    Lupton JR (2004) Microbial degradation products influence colon cancer risk: the butyrate controversy. J Nutr 134:479–482PubMedGoogle Scholar
  12. 12.
    Malinen E, Kassinen A, Rinttila T, Palva A (2003) Comparison of real-time PCR with SYBR Green I or 5′-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology 149:269–277CrossRefPubMedGoogle Scholar
  13. 13.
    Metchnikoff E (1907) The prolongation of life: optimistic studies. In: Mitchell C (ed) Lactic acid inhibiting intestinal putrefaction. William Heinemann, London, pp 161–183Google Scholar
  14. 14.
    Mitsuoka T (1992) Intestinal flora and aging. Nutr Rev 50:438–446CrossRefPubMedGoogle Scholar
  15. 15.
    Mueller S, Saunier K, Hanisch C, Norin E, Alm L, Midtvedt T, Cresci A, Silvi S, Orpianesi C, Verdenelli MC, Clavel T, Koebnick C, Zunft HJF, Doré J, Blaut M (2006) Differences in fecal microbiota in different European study populations in relation to age, gender, and country: a cross-sectional study. Appl Environ Microbiol 72:1027–1033CrossRefPubMedGoogle Scholar
  16. 16.
    Nadkarni MA, Martin FE, Jacques NA, Hunter N (2002) Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 148:257–266PubMedGoogle Scholar
  17. 17.
    Pryde SE, Duncan SH, Hold GL, Stewart CS, Flint HJ (2002) The microbiology of butyrate formation in the human colon. FEMS Microbiol Lett 217:133–139CrossRefPubMedGoogle Scholar
  18. 18.
    Rinttila T, Kassinen A, Malinen E, Krogius L, Palva A (2004) Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 97:1166–1177CrossRefPubMedGoogle Scholar
  19. 19.
    Stephen AM, Wiggins HS, Cummings JH (1987) Effect of changing transit time on colonic microbial metabolism in man. Gut 28:601–609CrossRefPubMedGoogle Scholar
  20. 20.
    Suau A, Bonnet R, Sutren M, Godon JJ, Gibson GR, Collins MD, Dore J (1999) Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65:4799–4807PubMedGoogle Scholar
  21. 21.
    Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP (2001) Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific pcr primers and denaturing gradient gel electrophoresis. Appl Environ Microbiol 67:2578–2585CrossRefPubMedGoogle Scholar
  22. 22.
    Woodmansey EJ (2007) Intestinal bacteria and ageing. J Appl Microbiol 102:1178–1186CrossRefPubMedGoogle Scholar
  23. 23.
    Woodmansey EJ, McMurdo MET, Macfarlane GT, Macfarlane S (2004) Comparison of compositions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects. Appl Environ Microbiol 70:6113–6122CrossRefPubMedGoogle Scholar
  24. 24.
    Zhang C, Zheng HT, Du XB, Zhang L, Zhu TX (1994) Initial studies between food compositions of Bama’s health macrobian and bifidobacteria in human intestinal tract. Food Sci China 177:47–49Google Scholar
  25. 25.
    Zou WZ (2002) Tabulation on the 2000 population census of Guangxi Zhuang autonomous region. Press of Statistics of China, BeijingGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Liang Zhao
    • 1
  • Wentao Xu
    • 1
  • Salam A. Ibrahim
    • 2
  • Junhua Jin
    • 1
  • Jiannan Feng
    • 1
  • Jingli Jiang
    • 3
  • Jianjun Meng
    • 4
  • Fazheng Ren
    • 1
    Email author
  1. 1.College of Food Science and Nutritional EngineeringChina Agricultural University (East Campus)BeijingChina
  2. 2.Food and Nutritional SciencesNorth Carolina A & T State UniversityGreensboroUSA
  3. 3.MengNiu Diary (Beijing) CompanyBeijingChina
  4. 4.Government of BaMa Yao Autonomous CountyGuangXi Zhuang Autonomous RegionChina

Personalised recommendations