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Current Microbiology

, Volume 62, Issue 1, pp 96–100 | Cite as

Cadmium Accumulation and Tolerance in Bradyrhizobium spp. (Peanut Microsymbionts)

  • Eliana Bianucci
  • Adriana Fabra
  • Stella CastroEmail author
Article

Abstract

In this study, the effect of cadmium (Cd) on cell viability and its accumulation in Bradyrhizobium spp. (peanut microsymbionts) as well as the role of glutathione (GSH) in the tolerance to this metal were investigated. A reference strain recommended as peanut inoculant (Bradyrhizobium sp. SEMIA6144) grew up to 10 μM Cd meanwhile a GSH-deficient mutant strain (Bradyrhizobium sp. SEMIA6144-S7Z) was unable to grow at this concentration. Two native peanut isolates obtained from Córdoba soils (Bradyrhizobium sp. NLH25 and Bradyrhizobium sp. NOD31) tolerated up to 30 μM Cd. The analysis of Cd content showed that Bradyrhizobium sp. SEMIA6144 accumulated a high amount of this metal, but a considerable inhibition of growth was observed compared to tolerant strains at 10 μM Cd. At this concentration, the intracellular GSH content of all the Bradyrhizobium sp. strains was not modified in comparison to control conditions. However, at 30 μM Cd, the intracellular GSH content significantly increased in Bradyrhizobium sp. strains NLH25 and NOD31. Thus, the distinct response of each Bradyrhizobium sp. strain to Cd reveals that, even in closely related lineages, there are strain-specific variations influencing the levels of tolerance to this metal. Indeed, the native peanut isolates tolerated higher Cd concentration than the reference strain, possibly due to an increase in GSH levels which could act as a detoxifying agent.

Keywords

Late Exponential Phase Tolerant Strain Peanut Kernel Subsistence Food Crop gshA Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgment

This research was supported by the SECyT-UNRC and CONICET, Argentina.

References

  1. 1.
    Anderson ME (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol 113:548–555CrossRefPubMedGoogle Scholar
  2. 2.
    Bååth E, Díaz-Ravina M, Frostegård Å, Campbell CD (1998) Effect of metal-rich sludge amendments on the soil microbial community. Appl Environ Microbiol 64:238–245PubMedGoogle Scholar
  3. 3.
    Bianucci E, Fabra A, Castro S (2008) Growth of Bradyrhizobium sp. SEMIA 6144 in response to methylglyoxal: role of glutathione. Curr Microbiol 56:371–375CrossRefPubMedGoogle Scholar
  4. 4.
    Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefPubMedGoogle Scholar
  5. 5.
    Cervantes C, Gutierrez Corona F (1994) Copper resistance mechanism in bacteria and fungi. FEMS Microbiol Rev 14:121–137CrossRefPubMedGoogle Scholar
  6. 6.
    Corticeiro SC, Lima AI, Figueira EM (2006) The importance of glutathione in oxidative status of Rhizobium lemuniosarum biovar viciae under Cd exposure. Enzym Microbial Technol 40:132–137CrossRefGoogle Scholar
  7. 7.
    Doyle J, Marshall R, Pfander W (1975) Effects of cadmium on the growth and uptake of cadmium by microorganisms. Appl Microbiol 29:562–564PubMedGoogle Scholar
  8. 8.
    Figueira EM, Lima AI, Pereira SI (2005) Cadmium tolerance plasticity in Rhizobium leguminosarum bv. viciae: glutathione as a detoxifing agent. Can J Microbiol 51:7–14CrossRefPubMedGoogle Scholar
  9. 9.
    Giller KE, Witter E, McGrath SP (1998) Toxicity of heavy metals to microorganisms and microbial process in agricultural soils: a review. Soil Biol Biochem 30:1389–1414CrossRefGoogle Scholar
  10. 10.
    Helbig K, Grosse C, Nies D (2008) Cadmium toxicity in glutathione mutants of Escherichia coli. J Bacteriol 190:5439–5454CrossRefPubMedGoogle Scholar
  11. 11.
    Inga C, Cabrera J, Spahn J, Tutti G, Badini R, Aguilar R, Martínez M, Casini C (2003) Valores bajos de Cd en el maní de la provincia de Córdoba. XVIII Jornada Nacional de Maní. Córdoba, Argentina, p 60Google Scholar
  12. 12.
    Khan M, Scullion J (2002) Effects of metal (Cd, Cu, Ni, Pb or Zn) enrichment of sewage-sludge on soil micro-organisms and their activities. Appl Soil Ecol 20:145–155CrossRefGoogle Scholar
  13. 13.
    Lakzian A, Murphy P, Turner A, Beynon JL, Giller KE (2002) Rhizobium leguminosarum bv. viciae populations in soils with increasing heavy metal contamination: abundance, plasmid profiles, diversity and metal tolerance. Soil Biol Biochem 34:519–529CrossRefGoogle Scholar
  14. 14.
    Levine WB, Marzluf GA (1989) Isolation and characterization of cadmium resistant mutant of Neurospora crassa. Can J Microbiol 35:359–365CrossRefPubMedGoogle Scholar
  15. 15.
    Matamoros MA, Moran JF, Iturbe-Ormaetxe I, Rubio MC, Becana M (1999) Glutathione and homoglutathione synthesis in legume root nodules. Plant Physiol 121:879–888CrossRefPubMedGoogle Scholar
  16. 16.
    McGrath SP, Chaudri AM, Giller KE (1995) Long-term effects of metals in sewage sludge on soils, microorganisms and plants. J Ind Microbiol 14:94–104CrossRefPubMedGoogle Scholar
  17. 17.
    Meister A (1988) Glutathione metabolism and its selective modification. J Biol Chem 263:17205–17210PubMedGoogle Scholar
  18. 18.
    Nies DH (2003) Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev 27:313–339CrossRefPubMedGoogle Scholar
  19. 19.
    Pacheco C, Passos J, Castro A, Moradas-Ferreira P, De Marco P (2008) Role of respiration and glutathione in cadmium-induced oxidative stress in Escherichia coli K-12. Arch Microbiol 189:271–278CrossRefPubMedGoogle Scholar
  20. 20.
    Pennincks MJ, Elskens MT (1993) Metabolism and functions of glutathione in microorganisms. Adv Microb Physiol 34:239–301CrossRefGoogle Scholar
  21. 21.
    Pereira SI, Lima AI, Figueira EM (2006) Screening possible mechanisms mediating cadmium resistance in Rhizobium leguminosarum bv. viciae isolated from contaminated portuguese soil. Microbial Ecol 52:176–186CrossRefGoogle Scholar
  22. 22.
    Purchase D, Miles R, Young T (1997) Cadmium uptake and nitrogen ability in heavy-metal resistant laboratory and field strains of Rhizobium leguminosarum biovar trifolii. FEMS Microbiol Ecol 22:85–93CrossRefGoogle Scholar
  23. 23.
    Riccillo PM, Muglia CI, De Bruijn FJ, Roe AJ, Booth IR, Aguilar OM (2000) Glutathione is involved in environmental stress responses in Rhizobium tropici, including acid tolerance. J Bacteriol 182:1748–1753CrossRefPubMedGoogle Scholar
  24. 24.
    Romeyer FM, Jacobs FA, Masson L, Hana Z, Brousseau R (1988) Bioaccumulation of heavy metals in Escherichia coli expressing an inducible synthetic human metallothionein gene. J Biotechnol 8:202–207CrossRefGoogle Scholar
  25. 25.
    Shi W, Bischoff M, Turco R, Konopka A (2002) Long-term effects of chromium and lead upon the activity of soil microbial communities. Appl Soil Ecol 21:169–177CrossRefGoogle Scholar
  26. 26.
    Silver S, Misra TK (1988) Plasmid-mediated heavy metal resistances. Annu Rev Microbiol 48:717–743CrossRefGoogle Scholar
  27. 27.
    Silver S, Phung LT (2005) A bacterial view of the periodic table: genes and proteins for toxic inorganic ions. J Ind Microbiol Biotechnol 32:587–605CrossRefPubMedGoogle Scholar
  28. 28.
    Siñeriz Louis M, Kothe E, Abate C (2009) Cadmium biosorption by Streptomyces sp. F4 isolated from former uranium mine. J Basic Microbiol 49:1–8CrossRefGoogle Scholar
  29. 29.
    Sobrevals L, Müller P, Fabra A, Castro S (2006) Role of glutathione in growth of Bradyrhizobium sp. (peanut microsymbiont) under different environmental stresses and in symbiosis with the host plant. Can J Microbiol 52:609–616CrossRefPubMedGoogle Scholar
  30. 30.
    Somasegaran P, Hoben H (1994) Quantifying the growth of rhizobia. In: Handbook for rhizobia. Springer, New York, pp 47–57Google Scholar
  31. 31.
    Taurian T, Aguilar OM, Fabra A (2002) Characterization of nodulating peanut rhizobia isolated from a native soil population in Córdoba, Argentina. Symbiosis 33:59–72Google Scholar
  32. 32.
    Vincent J (1970) A manual for the practical study of root nodule bacteria. In: International biological programme. Handbook no 15. Blackwell Scientific Publication, OxfordGoogle Scholar
  33. 33.
    Williams JW, Silver S (1984) Bacterial resistance and detoxification of heavy metals. Enzym Microbiol Technol 6:530–537CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Departamento de Ciencias Naturales, Facultad de Ciencias Exactas, Físico-Químicas y NaturalesUniversidad Nacional de Río CuartoRio CuartoArgentina

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