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Current Microbiology

, Volume 62, Issue 1, pp 90–95 | Cite as

Demonstration and Partial Characterization of a Bacterial Growth Enhancer in sera

  • Kanna OkayamaEmail author
  • Takeshi Honda
  • Shigeaki Matsuda
  • Tadashi Saito
  • Masaya Kawase
Article
  • 80 Downloads

Abstract

During our research into the pathogenesis of Vibrio parahaemolyticus, we noticed that the concentration of serum added to the tissue culture medium (Dulbecco’s modified Eagle’s medium: DMEM) greatly affected its growth. Using gel filtration column chromatography, we clearly demonstrated that serum contains not only a bacterial growth inhibitor (BGI) but also a bacterial growth enhancer (BGE) for Vibrio parahaemolyticus. Our data indicate that the BGI is transferrin, whereas the BGE seems to be an undescribed small molecule (molecular weight of 1,000–3,000 Da) and is associated with magnesium and molybdenum ions. BGE activity was not decreased by heat treatment (at 60 or 100°C for 30 min) and affected the growth rate of a wide range of Gram-negative and Gram-positive bacteria. The addition of EDTA into DMEM lowered the growth rate, whereas the addition of BGE restored the growth activity. This study suggests that sera contain a previously undescribed small BGE molecule.

Keywords

Bacterial Growth Inductively Couple Plasma Mass Spectrometry Enhance Activity Bacterial Growth Rate Bacterial Growth Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Grants-In-Aid for Scientific Research on Priority Areas and for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Kanna Okayama
    • 1
    • 2
    Email author
  • Takeshi Honda
    • 1
  • Shigeaki Matsuda
    • 3
  • Tadashi Saito
    • 4
  • Masaya Kawase
    • 5
  1. 1.Department of Bacterial Infections, Research Institute for Microbial DiseasesOsaka UniversitySuita, OsakaJapan
  2. 2.College of Nursing Art and ScienceUniversity of HyogoAkashiJapan
  3. 3.Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial DiseasesOsaka UniversitySuita, OsakaJapan
  4. 4.Radioisotope Research CenterOsaka UniversityToyonaka, OsakaJapan
  5. 5.Faculty of BioscienceNagahama Institute of Bio-science and TechnologyNagahama, ShigaJapan

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