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Current Microbiology

, Volume 62, Issue 1, pp 71–77 | Cite as

Diversity of Intestinal Bifidobacteria in Patients with Japanese Cedar Pollinosis and Possible Influence of Probiotic Intervention

  • Akira Kubota
  • Fang HeEmail author
  • Manabu Kawase
  • Gaku Harata
  • Masaru Hiramatsu
  • Hisakazu Iino
Article

Abstract

This study was conducted to evaluate the potential association between intestinal bifidobacteria and Japanese cedar pollinosis (JCPsis) and possible influences of probiotic intervention. In this study, fecal samples were the collected from 29 JCPsis patients. The qualitative and quantitative analyses of fecal bifidobacteria were conducted by quantitative real-time PCR with 16S rRNA-gene-targeted species-specific primers before cedar pollen spread and after a 10-week intervention with fermented milk prepared with Lactobacillus GG and L. gasseri TMC0356 during pollen spread. Each JCPsis patient had a unique diversity of bifidobacteria, which varied qualitatively and quantitatively in an individual-dependent manner during pollen spread. The serum IgE concentration of JCPsis patients with more than 3 detectable Bifidobacterium species was significantly lower than that of patients with less than 2 detected species. The prevalence of B. adolescentis, B. longum, and B. catenulatum increased after probiotic intervention, although the changes were not statistically significant. These results suggest that lower diversity of intestinal Bifidobacterium species might be a pathological aspect of JCPsis. The diversity of intestinal bifidobacteria could be a prospective target for using probiotics in the management of IgE-mediated allergic disorders including JCPsis.

Keywords

Fecal Sample Allergic Disease Intestinal Microbiota Fermented Milk Probiotic Intervention 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by Grant-in-Aid for Research and Development from the Japanese Ministry of Agriculture and Forestry.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Akira Kubota
    • 1
  • Fang He
    • 1
    Email author
  • Manabu Kawase
    • 1
  • Gaku Harata
    • 1
  • Masaru Hiramatsu
    • 1
  • Hisakazu Iino
    • 2
  1. 1.Technical Research LaboratoryTakanashi Milk Products Co., LtdYokohamaJapan
  2. 2.Graduate School, Science for Living SystemShowa Women’s UniversityTokyoJapan

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