Construction of 4″-Isovalerylspiramycin-I-Producing Strain by In-Frame Partial Deletion of 3-O-Acyltransferase Gene in Streptomyces spiramyceticus WSJ-1, the Bitespiramycin Producer
Bitespiramycin (BT), a multi-component antibiotic consisted mainly of 4″-isovalerylspiramycin I, II and III, is produced by Streptomyces spiramyceticus WSJ-1, a recombinant spiramycin-production strain that harbored the 4″-O-acyltransferase gene (ist) from Streptomyces mycarofaciens 1748, which could isovalerylate the 4″-OH of spiramycin. To eliminate the production of components 4″-isovalerylspiramycin II and III, therefore reducing the component complexity of BT, inactivation of the sspA gene, which encodes the 3-O-acyltransferase responsible for the acylation of spiramycin I to spiramycin II and III, was performed in Streptomyces spiramyceticus WSJ-1, by in-frame partial deletion. The resulting strain, Streptomyces spiramyceticus WSJ-2, is a 4″-isovalerylspiramycin-I-producing strain as expected.
KeywordsStreptomyces Minimal Inhibition Concentration Spiramycin Partial Deletion Apramycin
This research was supported by China National High Technology Programme (2006AA02Z230).
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