Current Microbiology

, Volume 62, Issue 1, pp 16–20 | Cite as

Construction of 4″-Isovalerylspiramycin-I-Producing Strain by In-Frame Partial Deletion of 3-O-Acyltransferase Gene in Streptomyces spiramyceticus WSJ-1, the Bitespiramycin Producer

  • Chunyan Ma
  • Hongxia Zhou
  • Jingyan Li
  • Jianlu Dai
  • Weiqing He
  • Hongyuan Wang
  • Linzhuan WuEmail author
  • Yiguang WangEmail author


Bitespiramycin (BT), a multi-component antibiotic consisted mainly of 4″-isovalerylspiramycin I, II and III, is produced by Streptomyces spiramyceticus WSJ-1, a recombinant spiramycin-production strain that harbored the 4″-O-acyltransferase gene (ist) from Streptomyces mycarofaciens 1748, which could isovalerylate the 4″-OH of spiramycin. To eliminate the production of components 4″-isovalerylspiramycin II and III, therefore reducing the component complexity of BT, inactivation of the sspA gene, which encodes the 3-O-acyltransferase responsible for the acylation of spiramycin I to spiramycin II and III, was performed in Streptomyces spiramyceticus WSJ-1, by in-frame partial deletion. The resulting strain, Streptomyces spiramyceticus WSJ-2, is a 4″-isovalerylspiramycin-I-producing strain as expected.


Streptomyces Minimal Inhibition Concentration Spiramycin Partial Deletion Apramycin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This research was supported by China National High Technology Programme (2006AA02Z230).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Chunyan Ma
    • 1
  • Hongxia Zhou
    • 1
  • Jingyan Li
    • 1
  • Jianlu Dai
    • 1
  • Weiqing He
    • 1
  • Hongyuan Wang
    • 1
  • Linzhuan Wu
    • 1
    Email author
  • Yiguang Wang
    • 1
    Email author
  1. 1.Key Lab of Biotechnology of Antibiotics, Ministry of Health, Institute of Medicinal BiotechnologyCAMS & PUMCBeijingChina

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