The use of food-grade microorganisms such as lactic acid bacteria (LAB) is one of the most promising methods for delivering health promoting compounds. Since it is not always possible to obtain strains that have the ability to produce specific compounds naturally or that produce them in sufficient quantities to obtain physiological responses, genetic modifications can be performed to improve their output. The objective of this study was to evaluate if previously studied genetically modified LAB (GM-LAB), with proven in vivo beneficial effects, are just as safe as the progenitor strain from which they were derived. Mice received an elevated concentration of different GM-LAB or the native parental strain from which they were derived during a prolonged period of time, and different health parameters were evaluated. Similar growth rates, hematological values, and other physiological parameters were obtained in the animals that received the GM-LAB compared to those that were fed with the native strain. These results demonstrate that the GM-LAB used in this study are just as safe as the native strains from which they were derived and thus merit further studies to include them into the food chain.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Brouwer IA, van Dusseldorp M, West CE et al (1999) Dietary folate from vegetables and citrus fruit decreases plasma homocysteine concentrations in humans in a dietary controlled trial. J Nutr 129:1135–1139
Hugenholtz J, Smid EJ (2002) Nutraceutical production with food-grade microorganisms. Curr Opin Biotechnol 13:497–507
Sybesma W, Hugenholtz J, de Vos WM et al (2006) Safe use of genetically modified lactic acid bacteria in food. Bridging the gap between consumers, green groups, and industry. Elect J Biotechnol 9:424–448
LeBlanc JG, Sybesma W, Starrenburg M et al (2010) Supplementation with engineered Lactococcus lactis improves the folate status in deficient rats. Nutrition 26
LeBlanc JG, Burgess C, Sesma F et al (2005) Ingestion of milk fermented by genetically modified Lactococcus lactis improves the riboflavin status of deficient rats. J Dairy Sci 88:3435–3442
LeBlanc JG, Ledue-Clier F, Bensaada M et al (2008) Ability of Lactobacillus fermentum to overcome host alpha-galactosidase deficiency, as evidenced by reduction of hydrogen excretion in rats consuming soya alpha-galacto-oligosaccharides. BMC Microbiol 8:22
LeBlanc JG, Piard JC, Sesma F et al (2005) Lactobacillus fermentum CRL 722 is able to deliver active alpha-galactosidase activity in the small intestine of rats. FEMS Microbiol Lett 248:177–182
Kuipers OP, de Ruyter PG, Kleerebezem M et al (1998) Quorum sensing-controlled gene expression in lactic acid bacteria. J Biotechnol 177:66–74
LeBlanc JG, Garro MS, Savoy de Giori G et al (2004) A Novel Functional Soy-based Food Fermented by Lactic Acid Bacteria: Effect of Heat Treatment. J Food Sci 69:M246–M250
Sainte-Marie G (1962) A paraffin embedding technique for studies employing immunofluorescence. J Histochem Cytochem 10:150–156
Wegmann U, O’Connell-Motherway M, Zomer A et al (2007) Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp. cremoris MG1363. J Bacteriol 189:3256–3270
Mierau I, Kleerebezem M (2005) 10 years of the nisin-controlled gene expression system (NICE) in Lactococcus lactis. Appl Microbiol Biotechnol 68:705–717
Nouaille S, Ribeiro LA, Miyoshi A et al (2003) Heterologous protein production and delivery systems for Lactococcus lactis. Genet Mol Res 2:102–111
Gasson MJ (1983) Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing. J Bacteriol 154:1–9
Sybesma W, Van Den Born E, Starrenburg M et al (2003) Controlled modulation of folate polyglutamyl tail length by metabolic engineering of Lactococcus lactis. Appl Environ Microbiol 69:7101–7107
Burgess C, O’Connell-Motherway M, Sybesma W et al (2004) Riboflavin production in Lactococcus lactis: potential for in situ production of vitamin-enriched foods. Appl Environ Microbiol 70:5769–5777
LeBlanc JG, Silvestroni A, Connes C et al (2004) Reduction of non-digestible oligosaccharides in soymilk: application of engineered lactic acid bacteria that produce alpha-galactosidase. Genet Mol Res 3:432–440
This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Consejo de Investigaciones de la Universidad Nacional de Tucumán (CIUNT) ECOS-Sud (Paris, France) and the European Commission through contract QLK1-CT-2000-01376 (Nutracells).
About this article
Cite this article
LeBlanc, J.G., Van Sinderen, D., Hugenholtz, J. et al. Risk Assessment of Genetically Modified Lactic Acid Bacteria Using the Concept of Substantial Equivalence. Curr Microbiol 61, 590–595 (2010). https://doi.org/10.1007/s00284-010-9657-7
- Lactic Acid Bacterium
- Genetically Modify Organism
- Native Strain
- Megaloblastic Anemia