Abstract
Percutaneous osseointegrated implant technology provides a potential alternative to current socket prosthetics for individuals with limb loss. However, similar to other percutaneous devices, there remain concerns of periprosthetic infection. To understand this process of infection, bacterial isolates were collected and characterized from a sheep model of osseointegration. CSA-13, a novel cationic steroid antimicrobial, was used at the skin/implant interface in an attempt to reduce the rate of infection. Results indicated that in this application, normal flora and environmental organisms continued to colonize the skin/implant interface as well as cause infection in the presence of CSA-13. Two factors are believed to have contributed to this outcome: the delivery of CSA-13 and the lack of a skin seal at the skin/implant interface, which would create a biological barrier to infection.
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Acknowledgments
This material is based upon work supported by the Office of Research and Development, Rehabilitation R&D Service, Department of Veterans Affairs, Salt Lake City, UT; the Albert and Margaret Hofmann Chair and the Department of Orthopaedics, University of Utah School of Medicine, Salt Lake City, UT. This research project is sponsored by the U.S. Department of the Army Award #W81XWH-05-1-0628. The U.S. Army Medical Research Acquisition Activity, 820 Chandler Street, Fort Detrick MD 21702-5014 is the awarding and administering acquisition office. Opinions, interpretations, conclusions, and recommendations are those of the author and are not necessarily endorsed by the U.S. Army. The content of this work does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred. Research was conducted in compliance with the Animal Welfare Act Regulations and other Federal statutes relating to animals and experiments involving animals and adheres to the principles set forth in the Guide for Care and Use of Laboratory Animals, National Research Council, 1996. The authors also acknowledge the technical help of those in the Research and Development, and Bacteriology sections of ARUP Laboratories.
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Williams, D.L., Bloebaum, R.D., Beck, J.P. et al. Characterization of Bacterial Isolates Collected from a Sheep Model of Osseointegration. Curr Microbiol 61, 574–583 (2010). https://doi.org/10.1007/s00284-010-9655-9
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DOI: https://doi.org/10.1007/s00284-010-9655-9