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Identification and Analysis of Polychlorinated Biphenyls (PCBs)-Biodegrading Bacterial Strains in Shanghai

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Abstract

As one of China’s great metropolises, Shanghai is vulnerable to various forms of industrial and agricultural contamination associated with its development. Polychlorinated biphenyls (PCBs) are man-made chemicals that never existed in nature until the 1900s when they started to be released into the environment. PCBs are hazardous environmental contaminants that bind strongly to soil. In this study, four soil samples were screened for the presence of PCB-degrading bacteria. The 16 S rDNAs were amplified from those genomes and the products (~1.5 kb) were purified and sequenced for the isolation and identification of bacterial species. Four Pseudomonas strains (strain 1-212 from sample 1; strain 2-241 from sample 2; strain 3-318 from sample 3; and strain 4-150 from sample 4) were selected for analysis by HPLC. Setting the content of the biphenyl in CK as 100%, the biphenyl contents was 2.32% in 1-212, 73.11% in 2-241, 69.83% in 3-318, and 86.16% in 4-150. The results of this study suggest directions for future research, including genetic screening, cloning and restructuring, and provide guidance for the cultivation of PCBs-degrading bacteria.

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References

  1. Faroon O, Keith L, Smith-Simon C, De Rosa C (2003) Polychlorinated biphenyls. Human health aspects. Concise international chemical assessment document 55. World Health Organization, Geneva

  2. Drinker CK, Warren MF, Bennett GA (1937) The problem of possible systemic effects from certain chlorinated hydrocarbons. J Ind Hyg Toxicol 19:283–311

    CAS  Google Scholar 

  3. Mayes BA, McConnell EE, Neal BH, Brunner MJ, Hamilton SB, Sullivan TM, Peters AC, Ryan MJ, Toft JD, Singer AW, Brown JF Jr, Menton RG, Moore JA (1998) Comparative carcinogenicity in Sprague-Dawley rats of the polychlorinated biphenyl mixtures Aroclors 1016, 1242, 1254, and 1260. Toxicol Sci 41:62–76

    CAS  PubMed  Google Scholar 

  4. Aoki Y (2001) Polychlorinated biphenyls, polychlorinated dibenzop-dioxins, and polychlorinated dibenzofurans as endocrine disrupters—what we have learned from Yusho disease. Environ Res 86:2–11

    Article  CAS  PubMed  Google Scholar 

  5. ATSDR (2000) Toxicological profile for polychlorinated biphenyls (PCBs). Agency for Toxic Substances and Disease Registry, US Department of Health and Human Services, Public Health Service, Atlanta

  6. Faroon O, Jones D, de Rosa C (2001) Effects of polychlorinated biphenyls on the nervous system. Toxicol Ind Health 16:305–333

    Article  CAS  PubMed  Google Scholar 

  7. Furukawa K, Matsumura F (1976) Microbial metabolism of polychlorinated biphenyls-studies on relative degradability of polychlorinated biphenyl components by Alcaligenes sp. J Agric Food Chem 24:251–256

    Article  CAS  PubMed  Google Scholar 

  8. Unterman R, Bedard DL, Brennan MJ, Bopp LH, Mondello FJ, Books RE, Mobley DP, McDermott JB, Schwartz CC, Dietric DK (1988) Biological approaches for polychlorinated biphenyl degradation. In: Omenn GS (ed) Environmental biotechnology: reducing risks from environmental chemicals through biotechnology. Plenum Press, New York

    Google Scholar 

  9. Abramowicz DA (1990) Aerobic and anaerobic biodegradation of PCBs: A Review. Crit Rev Biotechnol 10:241–251

    Article  CAS  Google Scholar 

  10. Furukawa K (1982) Microbial degradation of polychlorinated biphenyls. In: Chakrabarty AM (ed) Biodegradation and detoxification of environmental pollutants. CRC Press, Boca Raton

    Google Scholar 

  11. Furukawa K (1994) Molecular genetics and evolutionary relationship of PCB-degrading bacteria. Biodegradation 5:289–300

    Article  CAS  PubMed  Google Scholar 

  12. Unterman R (1996) A history of PCBs biodegradation. In: Cawford RL, Crawford RL, Crawford DL (eds) Bioremediation:principles and applications. University Press, New York, Cambridge

    Google Scholar 

  13. Furukawa K, Fujihara H (2008) Microbial degradation of polychlorinated biphenyls: biochemical and molecular features. J Biosci Bioeng 105:433–449

    Article  CAS  PubMed  Google Scholar 

  14. Hong Q, Dong XJ, He LJ, Li SP (2009) Isolation of a biphenyl-degrading bacterium, Achromobacter sp. BP3, and cloning of the bph gene cluster. Int Biodeter Biodegr 63:365–370

    Article  CAS  Google Scholar 

  15. Erickson BD, Mondello FJ (1992) Nucleotide sequencing and transcriptional mapping of the genes encoding biphenyl dioxygenase, a multicomponent polychlorinated-biphenyl-degrading enzyme in Pseudomonas strain LB400. J Bacteriol 174:2903–2912

    CAS  PubMed  Google Scholar 

  16. Hofer B, Backhaus S, Timmis KN (1994) The biphenyl/polychlorinated biphenyl-degradation locus (bph) of Pseudomonas sp. LB400 encodes four additional metabolic enzymes. Gene 144:9–16

    Article  CAS  PubMed  Google Scholar 

  17. Furukawa K, Miyazaki T (1986) Cloning of a gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligenes. J Bacteriol 166:392–398

    CAS  PubMed  Google Scholar 

  18. Kimbara K, Hashimoto T, Fukuda M, Koana T, Takagi M, Oishi M, Yano K (1989) Cloning and sequencing of two tandem genes involved in degradation of 2, 3-dihydroxybiphenyl to benzoic acid in the polychlorinated biphenyl-degrading soil bacterium Pseudomonas sp. strain KKS102. J Bacteriol 171:2740–2747

    CAS  PubMed  Google Scholar 

  19. Kikuchi Y, Yasukochi Y, Nahata Y, Fukuda M, Takagi M (1994) Nucleotide sequence and functional analysis of the metacleavage pathway involved in biphenyl and polychlorinated biphenyl degradation in Pseudomonas sp. strain KKS102. J Bacteriol 176:4269–4276

    CAS  PubMed  Google Scholar 

  20. Springael D, Kreps S, Mergeay M (1993) Identification of a catabolic transposon, Tn4371, carrying biphenyl and 4-chlorobiphenyl degradation genes in Alcaligenes eutropus A5. J Bacteriol 175:1674–1681

    CAS  PubMed  Google Scholar 

  21. Merlin C, Springael D, Mergeay M, Toussaint A (1997) Organization of the bph gene cluster of transposon Tn4371, encoding enzymes for the degradation of biphenyl and 49 chlorobiphenyl compounds. Mol Gen Genet 253:499–506

    Article  CAS  PubMed  Google Scholar 

  22. Mouz S, Merlin C, Springael D, Toussaint A (1999) A GntR-like negative regulator of the biphenyl degradation genes of the transposon Tn4371. Mol Gen Genet 262:790–799

    Article  CAS  PubMed  Google Scholar 

  23. Masai E, Yamada A, Healy JM, Hatta T, Kinbara K, Fukuda M, Yano K (1995) Characterization of biphenyls catabolic genes of gram-positive polychlorinated biphenyls degrader Rhodococcus sp. strain RHA1. Appl Environ Microbiol 61:2079–2085

    CAS  PubMed  Google Scholar 

  24. Yang X, Liu X, Xie F, Zhang G, Qian S (2007) Characterization and functional analysis of a novel gene cluster involved in biphenyl degradation in Rhodococcus sp. strain R04. J Appl Microbiol 103:2214–2224

    Article  CAS  PubMed  Google Scholar 

  25. Taguchi K, Motoyama M, Lida T, Kudo T (2007) Polychlorinated biphenyl/biphenyl degrading gene clusters in Rhodococcus sp. K37, HA99, and TA431 are different from well-known bph gene clusters of Rhodococci. Biosci Biotechnol Biochem 71:1136–1144

    Article  CAS  PubMed  Google Scholar 

  26. Yoon JH, Lee ST, Kim SB, Kim WY, Goodfellow M, Park YH (1997) Restriction fragment length polymorphisms analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 47:111–114

    Article  CAS  Google Scholar 

  27. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis MEGA software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  28. Abramowicz DA (1995) Aerobic and anaerobic PCB biodegradation in the environment. Environ Health Perspect 103(5):97–99

    Article  CAS  PubMed  Google Scholar 

  29. Mills SA 3rd, Thal DI, Barney J (2007) A summary of the 209 PCB congener nomenclature. Chemosphere 68:1603–1612

    Article  CAS  PubMed  Google Scholar 

  30. Field JA, Sierra-Alvarez R (2008) Microbial transformation and degradation of polychlorinated biphenyls. Environ Pollut 155:1–12

    Article  CAS  PubMed  Google Scholar 

  31. Cheng JP, Wu Q, Xie HY, Gu JM, Zhao WC, Ma J, Wang WH (2007) Polychlorinated biphenyls (PCBs) in PM10 surrounding a chemical industrial zone in Shanghai, China. Bull Environ Contam Toxicol 79:448–453

    Article  CAS  PubMed  Google Scholar 

  32. Ma J, Cheng J, Xie H, Hu X, Li W, Zhang J, Yuan T, Wang W (2007) Seasonal and spatial character of PCBs in a chemical industrial zone of Shanghai, China. Environ Geochem Health 29:503–511

    Article  CAS  PubMed  Google Scholar 

  33. Sylvestre M, Macek T, Mackova M (2009) Transgenic plants to improve rhizoremediation of polychlorinated biphenyls (PCBs). Curr Opin Biotechnol 20:242–247

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

The research was supported by the Hi-tech Research and Development 863 Program of China (2008AA10Z401); Shanghai Rising-Star Program and Natural Science Foundation (08QH14021).

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Authors and Affiliations

  1. College of Bioscience and Biotechnology, Yangzhou University, 88 Daxue Road, Yangzhou, 225009, China

    Jian-Jun Shuai & Fei Xiong

  2. Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Rd, Shanghai, China

    Jian-Jun Shuai, Yong-Sheng Tian, Quan-Hong Yao, Ri-He Peng & Ai-Sheng Xiong

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  1. Jian-Jun Shuai
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  2. Yong-Sheng Tian
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  3. Quan-Hong Yao
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  4. Ri-He Peng
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  6. Ai-Sheng Xiong
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Correspondence to Fei Xiong or Ai-Sheng Xiong.

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Jian-Jun Shuai and Yong-Sheng Tian are contributed equally to the article.

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Shuai, JJ., Tian, YS., Yao, QH. et al. Identification and Analysis of Polychlorinated Biphenyls (PCBs)-Biodegrading Bacterial Strains in Shanghai. Curr Microbiol 61, 477–483 (2010). https://doi.org/10.1007/s00284-010-9641-2

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  • DOI: https://doi.org/10.1007/s00284-010-9641-2

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