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Involvement of recF in 254 nm Ultraviolet Radiation Resistance in Deinococcus radiodurans and Escherichia coli

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Abstract

recF is a critical gene involved in the RecFOR pathway of DNA repair in Deinococcus radiodurans (D. radiodurans). To investigate the role of recF in UV-C radiation resistance, we generated the recF-deficient D. radiodurans strain R1, expressed recF in Escherichia coli (E. coli) BL21 cells, and compared the ability of each to resist UV-C radiation by F10, inactivation constant (IC), and extrapolation number (N). The mutation of recF in D. radiodurans R1 resulted in characteristic slow growth and dramatic sensitivity to UV-C irradiation. Transformation of recF into E. coli BL21 cells resulted in increased UV-C resistance compared to untransformed E. coli BL21 cells. These results suggested that recF is needed for the replication of D. radiodurans. Furthermore, as a part of the RecFOR pathway in D. radiodurans, disruption of recF could dramatically decrease the UV-C resistance of D. radiodurans and recF could increase the UV-C resistance of E. coli BL21 cells.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (30670635 and 50804049), the Medical and Health Foundation of the PLA (06M0199) and Natural Science Foundation of Chongqing China (CSTC2007BB5004).

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Authors and Affiliations

  1. Department of Environmental Hygiene, Third Military Medical University, Gaotanyan Street 30, Chongqing, 400038, China

    Xiaosong Chang, Lan Yang, Qing Zhao, Wenjuan Fu, Hao Chen, Zhiqun Qiu, Ji-an Chen, Ran Hu & Weiqun Shu

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  1. Xiaosong Chang
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  2. Lan Yang
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Correspondence to Weiqun Shu.

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Chang, X., Yang, L., Zhao, Q. et al. Involvement of recF in 254 nm Ultraviolet Radiation Resistance in Deinococcus radiodurans and Escherichia coli . Curr Microbiol 61, 458–464 (2010). https://doi.org/10.1007/s00284-010-9638-x

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  • DOI: https://doi.org/10.1007/s00284-010-9638-x

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