Current Microbiology

, Volume 61, Issue 5, pp 422–428 | Cite as

Prevention of Chinese Sacbrood Virus Infection in Apis cerana using RNA Interference

Article

Abstract

Chinese sacbrood virus (CSBV) is the pathogen of Chinese sacbrood disease, which poses a serious threat to honeybee Apis cerana, and tends to cause bee colony and even the whole apiary collapse. Here we report on prevention of CSBV infection by feeding second instar larvae of A. cerana with specific sequences of CSBV double-stranded RNA (dsRNA). Protection of the bee larvae from CSBV by ingestion of CSBV-derived dsRNA was further demonstrated by quantitative real-time PCR (qRT-PCR) and northern blot analysis. The result provides a potential method to protect A. cerana from CSBV infection.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.South China Botanical Garden, Chinese Academy of SciencesGuangzhouChina
  2. 2.Guangdong Entomological InstituteGuangzhouChina
  3. 3.Sun Yat-sen UniversityGuangzhouChina

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