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iTRAQ-Coupled 2D LC–MS/MS Analysis of Secreted Proteome of HBV-Replicating HepG2 Cells: Potential in Biomarkers for Prognosis of HCC

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Abstract

Hepatitis B virus (HBV) infection remains a major health concern with more than 350 million carriers in the world. It is associated with acute and chronic liver diseases including hepatocellular carcinoma (HCC). The early detection of severe liver diseases related to HBV is crucial for the effective treatment. This work aims to investigate the secreted proteins in our recently established cell-based HBV replication system, using isobaric tags for relative and absolute quantitation (iTRAQ)-coupled 2D LC–MS/MS proteomics approach. Such proteins are reflective of early events of HBV infection and thus may have potential as prognostic biomarkers for development of liver diseases.

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Acknowledgments

This work was supported by Grant RG42/05 (WN Chen). Huixing Feng and Mingxuan Wang are recipients of graduate research scholarship from Nanyang Technological University, Singapore. We thank Jianjun Sui for technical assistance.

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Authors and Affiliations

  1. School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Singapore

    Huixing Feng, Mingxuan Wang & Wei Ning Chen

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  1. Huixing Feng
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  2. Mingxuan Wang
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  3. Wei Ning Chen
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Correspondence to Wei Ning Chen.

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Feng, H., Wang, M. & Chen, W.N. iTRAQ-Coupled 2D LC–MS/MS Analysis of Secreted Proteome of HBV-Replicating HepG2 Cells: Potential in Biomarkers for Prognosis of HCC. Curr Microbiol 61, 280–284 (2010). https://doi.org/10.1007/s00284-010-9608-3

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  • DOI: https://doi.org/10.1007/s00284-010-9608-3

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