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Identification of Actinomycetes Producing Phospholipase D with High Transphosphatidylation Activity

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Abstract

Previously we isolated six actinomycetes strains, 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4, that produce phospholipase D (PLD) with high transphosphatidylation activity. In this study, we identified these strains, and the PLD activities were compared with those of reference strains. 16S rDNA sequences and DNA–DNA hybridization tests indicated taxonomic affiliations of strain 9-6 with Streptomyces senoensis, strains 10-1 and 10-6 with S. vinaceus, and strains 10-2 and 10-3 with S. racemochromogenes. Strain 21-4, though identified as a Streptomyces sp., could not be identified with any known species. Meanwhile, most of the culture supernatants of reference strains demonstrated no or very weak PLD activity, while those of our strains exhibited significantly higher activity. All of the strains in this study were identified as Streptomyces species. The PLD activity of our strains exceeded most of the reference Streptomyces strains. The findings in this study imply that the Streptomyces strains, although they are members of the same species, can produce different quantities of PLD enzyme.

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Acknowledgments

The authors are grateful to Prof. Toshio Nagashima, Tokyo University of Agriculture for his excellent contributions to this work.

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Authors and Affiliations

  1. Faculty of Bioindustry, Department of Food Science and Technology, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido, 099-2493, Japan

    Yozo Nakazawa, Takeshi Nagai & Hiroaki Sato

  2. Faculty of Applied Bioscience, Department of Applied Biology and Chemistry, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-8502, Japan

    Rei Suzuki, Masataka Uchino & Katsumi Takano

  3. Sars International Centre for Marine Molecular Biology, Bergen High Technology Centre, Thormøhlensgt. 55, 5008, Bergen, Norway

    Yoshimasa Sagane

  4. Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Takuji Kudo

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  1. Yozo Nakazawa
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  2. Rei Suzuki
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  3. Masataka Uchino
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  4. Yoshimasa Sagane
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  5. Takuji Kudo
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  6. Takeshi Nagai
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  7. Hiroaki Sato
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  8. Katsumi Takano
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Corresponding author

Correspondence to Yozo Nakazawa.

Additional information

Nucleotide sequences

The DDBJ/EMBL/GenBank accession numbers for 16S rDNA sequences of strain 9-4, 10-1, 10-2, 10-3, 10-6, and 21-4 are AB222067, AB222068, AB222070, AB222071, AB222069, and AB222072, respectively.

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Nakazawa, Y., Suzuki, R., Uchino, M. et al. Identification of Actinomycetes Producing Phospholipase D with High Transphosphatidylation Activity. Curr Microbiol 60, 365–372 (2010). https://doi.org/10.1007/s00284-009-9551-3

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