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Yeast Microflora Isolated From Brazilian Cassava Roots: Taxonomical Classification Based on Molecular Identification

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Abstract

A rapid molecular identification technique was applied on microbial microflora isolated from Brazilian cassava roots given a yeast profile presented in the samples analyzed. A total of 24 strain isolated from cassava were initially grouped and identified in five groups using restriction-fragment length polymorphism (RFLPs) of 5.8S-ITS rDNA region. Sequencing analysis of the domains D1 and D2 of the 26S rRNA gene or 5.8S rRNA-ITS region were used to identify different groups of yeasts. Representative colonies of yeasts of each group were isolated and identified as Debaromyces hansenii, Kodamaea ohmeri, Candida glabrata, C. haemulonii, and Pichia gullhermondii. It is hoped that these results will contribute toward selecting yeast from this microflora capable to degrade cassava starch in the near future.

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Acknowledgment

This work was supported by CSIC/IATA and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Universidade Federal do Pará, Laboratório de Investigação Sistemática em Biotecnologia e Química Fina, Belém, Brazil

    Nelson Ferreira & Alberdan Santos

  2. Instituto de Agroquimica y Tecnologia de Alimentos, Biotecnología, Valencia, Spain

    Carmela Belloch, Amparo Querol, Paloma Manzanares & Salvador Vallez

Authors
  1. Nelson Ferreira
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  2. Carmela Belloch
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  3. Amparo Querol
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  4. Paloma Manzanares
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  5. Salvador Vallez
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  6. Alberdan Santos
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Corresponding authors

Correspondence to Nelson Ferreira or Carmela Belloch.

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Ferreira, N., Belloch, C., Querol, A. et al. Yeast Microflora Isolated From Brazilian Cassava Roots: Taxonomical Classification Based on Molecular Identification. Curr Microbiol 60, 287–293 (2010). https://doi.org/10.1007/s00284-009-9539-z

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  • DOI: https://doi.org/10.1007/s00284-009-9539-z

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