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Expression and Characterization of Penicillin-Binding Proteins in Burkholderia cenocepacia

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Abstract

Putative penicillin-binding proteins (PBPs) were identified in the genome of the Burkholderia cenocepacia strain J2315 based on homology to E. coli PBPs. The three sequences identified as homologs of E. coli PBP1a, BCAL2021, BCAL0274, and BCAM2632, were cloned and expressed as His6-tagged fusion proteins in E. coli. The fusion proteins were isolated and shown to bind β-lactams, indicating these putative PBPs have penicillin-binding activity.

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Acknowledgments

This work was supported by funding from the Denison University Research Foundation and a Camille and Henry Dreyfus Foundation Faculty Start-up Award for KMS.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Denison University, Granville, USA

    Kimberly Musa Specht, Kyle H. Sheetz, Courtney M. Alexander, Lilian T. Lamech, Lauren H. O’Connor, Dawn M. Walker & Hilary P. Stevenson

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  1. Kimberly Musa Specht
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  2. Kyle H. Sheetz
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Correspondence to Kimberly Musa Specht.

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Specht, K.M., Sheetz, K.H., Alexander, C.M. et al. Expression and Characterization of Penicillin-Binding Proteins in Burkholderia cenocepacia . Curr Microbiol 60, 274–279 (2010). https://doi.org/10.1007/s00284-009-9537-1

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