Current Microbiology

, Volume 60, Issue 3, pp 185–190 | Cite as

Potentially Pathogenic Acanthamoeba Isolated from a Hospital in Brazil

  • Ana Maris Carlesso
  • Geórgia Lazzari Artuso
  • Karin Caumo
  • Marilise Brittes RottEmail author


Studies on free-living amoebae (FLA), has been increased in recent years, especially related to the genus Acanthamoeba, because these organisms are widely found in the environment. The present work isolated and characterized this organism from biofilms and dust in hospital environment. 135 samples were collected in 15 different environments in a hospital at the south of Brazil. Thirty-one (23%) isolates were identified as morphologically belonging to the Acanthamoeba genus and 10 of these were submitted to temperature and osmotolerance tests as criterion for evaluation of the viability and pathogenicity. The tests indicate that four (40%) of these isolates could be potentially pathogenic because grew at high temperature (40°C) and osmolarity (mannitol 1 M). Some isolates genotypes were determined after ribosomal DNA sequencing. These data revealed that three dust isolates belong to T4, two biofilm isolates to T5 and one dust isolate to T3 genotype. Therefore, Acanthamoeba found in the hospital environment represents a risk for people that circulate there.


Hospital Environment Acanthamoeba Keratitis Monoxenic Culture Acanthamoeba Isolate Drinking Fountain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ana Maris Carlesso
    • 1
  • Geórgia Lazzari Artuso
    • 1
  • Karin Caumo
    • 2
  • Marilise Brittes Rott
    • 3
    Email author
  1. 1.Universidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.PPG Microbiologia Agrícola e do AmbienteUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Department of MicrobiologyUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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