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Expression and Purification of an Antimicrobial Peptide, Bovine Lactoferricin Derivative LfcinB-W10 in Escherichia coli

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Abstract

Antimicrobial peptides (AMPs) are extremely attractive candidate for therapeutic agents due to their wide spectrum of antimicrobial activity and action mechanism different from antibiotics. In this study, a method using genetic engineering for obtaining an antimicrobial peptide, bovine lactoferricin derivative peptide LfcinB-W10, has been developed. According to the coden usage of Escherichia coli, a gene encoding the peptide was synthesized and a recombinant vector of E. coli expression pGEX-EN-LFW was constructed. The LfcinB-W10 peptide fused with glutathione S-transferase (GST) was successfully expressed and about 20 mg fusion protein with 90% purity was obtained from 1 l culture. The recombinant LfcinB-W10 (rLfcinB-W10) was released from fusion protein by the enterokinase digestion, and about the LfcinB-W10 yield reached 300 μg per 1 l culture. The purified rLfcinB-W10 was found to have growth inhibition activity against Staphylococcus aureus (S. aureus) ATCC25923.

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Acknowledgements

This work was supported by the grants from National Natural Science Foundation of China (No. 30800794), Youth Science Funds of Heillongjiang Province (No. QC08C05) and Research Foundation of Northeast Agricultural University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin, 150030, China

    Xingjun Feng, Jiayin Guo, Chongpeng Bi, Baojing Cheng, Zhongyu Li & Anshan Shan

  2. Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin, 150081, China

    Chunlong Liu

  3. Animal Science Research Centre, Hei Long Jiang Academy of Agricultural Research, Harbin, 150086, China

    Zhongqiu Li

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  1. Xingjun Feng
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  2. Chunlong Liu
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  3. Jiayin Guo
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  4. Chongpeng Bi
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  5. Baojing Cheng
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  6. Zhongyu Li
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  7. Anshan Shan
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  8. Zhongqiu Li
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Corresponding authors

Correspondence to Xingjun Feng or Anshan Shan.

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Xingjun Feng and Chunlong Liu contributed equally to this work.

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Feng, X., Liu, C., Guo, J. et al. Expression and Purification of an Antimicrobial Peptide, Bovine Lactoferricin Derivative LfcinB-W10 in Escherichia coli . Curr Microbiol 60, 179–184 (2010). https://doi.org/10.1007/s00284-009-9522-8

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  • DOI: https://doi.org/10.1007/s00284-009-9522-8

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