Abstract
Xylitol consumption decreases counts of mutans streptococci. However, the mechanism behind this decrease is not well understood. We studied not only type strains and clinical isolates of mutans streptococci, but also other polysaccharide-forming oral streptococci. Growth inhibition and adherence of cells to a smooth glass surface—reflecting synthesis of water-insoluble polysaccharides were studied in the presence of 2% (0.13 mol/l) and 4% (0.26 mol/l) xylitol. The effect of xylitol was compared to a novel polyol sweetener, erythritol. Except for Streptococcus mutans 10449 and S. sobrinus OMZ 176, the glass surface adhesion of most polysaccharide-forming streptococci was reduced by the presence of both 4% xylitol and erythritol. For the S. mutans and S. sobrinus type strains, the growth inhibition with 4% xylitol and erythritol was 36–77% and for the clinical S. mutans isolates 13–73%. Of the other oral streptococci, only S. sanguinis was inhibited with 4% xylitol (45–55%). For both polyols, the magnitude of the growth inhibition observed was not associated with the magnitude of the decrease in adherence (xylitol: r = −0.18; erythritol: r = 0.49). In conclusion, both xylitol and erythritol can decrease polysaccharide-mediated cell adherence contributing to plaque accumulation through a mechanism not dependent on growth inhibition.
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References
Banas JA, Vickerman MM (2003) Glucan-binding proteins of the oral streptococci. Crit Rev Oral Biol Med 14:89–99
Beighton D (2005) The complex oral microflora of high-risk individuals and groups and its role in the caries process. Community Dent Oral Epidemiol 33:248–255
Bradshaw DJ, Marsh PD (1994) Effect of sugar alcohols on the composition and metabolism of a mixed culture of oral bacteria grown in a chemostat. Caries Res 28:251–256
Holgerson PF, Stecksén-Blicks C, Sjöström I, Öberg M, Twetman S (2006) Xylitol concentration in saliva and dental plaque after use of various xylitol-containing products. Caries Res 40:393–397
Knuuttila ML, Mäkinen KK (1975) Effect of xylitol on the growth and metabolism of Streptococcus mutans. Caries Res 9:177–189
Kontiokari T, Uhari M, Koskela M (1995) Effect of xylitol on growth of nasopharyngeal bacteria in vitro. Antimicrob Agents Chemother 39:1820–1823
Loesche WJ, Grossman NS, Earnest R, Corpron R (1984) The effect of chewing xylitol gum on the plaque and saliva levels of Streptococcus mutans. J Am Dent Assoc 108:587–592
Ly KA, Milgrom P, Rothen M (2006) Xylitol, sweeteners, and dental caries. Pediatr Dent 28:154–163
Mäkinen KK, Isotupa KP, Kivilompolo T, Mäkinen P-L, Murtomaa S, Petäjä J, Toivanen J, Söderling E (2002) The effect of polyol-combinant saliva stimulants on S. mutans levels in plaque and saliva of patients with mental retardation. Spec Care Dent 22:187–193
Mäkinen KK, Isotupa KP, Kivilompolo T, Mäkinen P-L, Toivanen J, Söderling E (2001) Comparison of erythritol and xylitol saliva stimulants in the control of dental plaque and mutans streptococci. Caries Res 35:129–135
Mäkinen KK, Saag M, Isotupa KP, Olak J, Nommela R, Söderling E, Mäkinen P-L (2005) Similarity of the effects of erythritol and xylitol on some risk factors of dental caries. Caries Res 39:207–215
Mäkinen KK, Söderling E, Hurttia H, Lehtonen OP, Luukkala E (1985) Biochemical, microbiologic, and clinical comparisons between two dentifrices that contain different mixtures of sugar alcohols. J Am Dent Assoc 111:745–751
Mattos-Graner RO, Smith DJ, King WF, Mayer MPA (2000) Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12- to 30-month-old children. J Dent Res 79:1371–1377
Milgrom P, Ly KA, Roberts MC, Rothen M, Mueller G, Yamaguchi DK (2006) Mutans streptococci dose response to xylitol chewing gum. J Dent Res 85(2):177–181
Miyasawa-Hori H, Aizawa S, Takahashi N (2006) Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains and the biochemical mechanism. Oral Microbiol Immunol 21:201–205
Rogers AH, Pilowsky KA, Zilm PS, Gully NJ (1991) Effects of pulsing with xylitol on mixed continuos cultures of oral streptococci. Aust Dent J 36:231–235
Söderling E (2009) Xylitol, mutans streptococci, and dental plaque. Adv Dent 21:74–78
Söderling E, Alaräisänen L, Scheinin A, Mäkinen KK (1987) Effect of xylitol and sorbitol on polysaccharide production by and adhesive properties of Streptococcus mutans. Caries Res 21:109–116
Söderling EM, Ekman TC, Taipale TJ (2008) Growth inhibition of Streptococcus mutans with low xylitol concentrations. Curr Microbiol 56:382–385
Takahashi N, Nyvad B (2008) Caries ecology revisited: microbial dynamics and the caries process. Caries Res 42:409–418
Tanzer JM, Livingston J, Thompson AM (2001) The microbiology of primary dental caries in humans. J Dent Educ 65:1028–1037
Trahan L (1995) Xylitol: a review of its action on mutans streptococci and dental plaque—its clinical significance. Int Dent J 45(1 Suppl 1):77–92
Vadeboncoeur C, Trahan L, Mouton C, Mayrand D (1983) Effect of xylitol on the growth and glycolysis of acidogenic oral bacteria. J Dent Res 62:82–884
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Söderling, E.M., Hietala-Lenkkeri, AM. Xylitol and Erythritol Decrease Adherence of Polysaccharide-Producing Oral Streptococci. Curr Microbiol 60, 25–29 (2010). https://doi.org/10.1007/s00284-009-9496-6
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DOI: https://doi.org/10.1007/s00284-009-9496-6