Cryptococcus shivajii sp. nov.: A Novel Basidiomycetous Yeast Isolated from Biogas Reactor

Abstract

Five yeast morphotypes were isolated from biogas reactors at North Wyke Research, Okehampton, UK. Out of the five morphotypes, four were identified as known species. In contrast, the fifth morphotype strain, Bio10T, was found to differ from Bullera dendrophila and Kwoniella mangroviensis, its closest phylogenetic neighbours, by 2.6–2.9% with respect to the nucleotide sequence of the D1/D2 domain of the 26S rRNA gene and by 5.6–6.2% with respect to the internal transcribed spacer 1 (ITS1)-5.8S rRNA gene-ITS2 region. Bio10T also differs from these two species by a number of phenotypic characteristics. Thus, based on the phenotypic differences and phylogenetic analysis, strain Bio10T is assigned the status of a new species of Cryptococcus, for which the name Cryptococcus shivajii sp. nov. is proposed. The type strain is Bio10T (NCYC 3541T = CBS 11374T).

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Acknowledgements

We would like to thank Biotechnology and Biological Sciences Research Council (BBSRC) UK for India Partnering Award and EU for Agro biogas project. North Wyke Research is an institute of the Biotechnology and Biological Sciences Research Council of UK. We thank Dr Mary Parker for her kind advice and assistance with the imaging at the Institute of Food Research, Norwich Research Park, Colney, Norwich, UK. Sreenivas would like to thank Waqar Ali, University of Oxford, UK for his bioinformatics support during bioinformatics course at University of Oxford, UK.

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Correspondence to Phil J. Hobbs.

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Ravella, S.R., James, S.A., Bond, C.J. et al. Cryptococcus shivajii sp. nov.: A Novel Basidiomycetous Yeast Isolated from Biogas Reactor. Curr Microbiol 60, 12–16 (2010). https://doi.org/10.1007/s00284-009-9493-9

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Keywords

  • Biogas
  • Anaerobic Digestion
  • Rose Bengal
  • Corn Meal Agar
  • Rhodotorula Glutinis