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Cys92, Cys101, Cys197, and Cys203 Are Crucial Residues for Coordinating the Iron–Sulfur Cluster of RhdA from Acidithiobacillus ferrooxidans

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Abstract

By proteomic analysis, we found a rhodanese-like protein(RhdA) from Acidithiobacillus ferrooxidans ATCC 23270 whose C-terminal contained a cysteine motif (Cys-XX-Trp-XX-Cys), known to bind iron–sulfur clusters. But so far, there were no articles to confirm the existence of iron–sulfur cluster in RhdA. In this study, RhdA gene from A. ferrooxidans ATCC 23270 was cloned and expressed in Escherichia coli, the protein was purified by one-step affinity chromatography to homogeneity. The UV–Vis scanning and EPR spectra results indicated that the wild-type proteins contained an iron–sulfur cluster. Site-directed mutagenesis results revealed that the four cysteines Cys92, Cys101, Cys197, and Cys203 were crucial residues for iron–sulfur cluster binding.

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Acknowledgments

This work was supported by the National Basic Research Program of P.R. China (2004CB619204), National Natural Science Foundation of P.R.China (50621063, 50874032) and Foundation for the Author of National Excellent Doctoral Dissertation of P.R. China (200549).

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Authors and Affiliations

  1. Department of Bioengineering, School of Resources Processing and Bioengineering, Central South University, 410083, Changsha, Hunan, People’s Republic of China

    Yunjie Dai, Chunli Zheng, Anna Wu, Jia Zeng & Guanzhou Qiu

  2. Department of Environmental Science & Engineering, Donghua University, 201620, Shanghai, People’s Republic of China

    Jianshe Liu

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  1. Yunjie Dai
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  3. Chunli Zheng
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Correspondence to Jianshe Liu.

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Dai, Y., Liu, J., Zheng, C. et al. Cys92, Cys101, Cys197, and Cys203 Are Crucial Residues for Coordinating the Iron–Sulfur Cluster of RhdA from Acidithiobacillus ferrooxidans . Curr Microbiol 59, 559–564 (2009). https://doi.org/10.1007/s00284-009-9476-x

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  • DOI: https://doi.org/10.1007/s00284-009-9476-x

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