Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties of Rose Essential Oil, Hydrosol and Absolute


The antioxidant and antibacterial activities, and total phenolic contents of Rosa damascena Mill. flower extracts (absolute, essential oil and hydrosol) were investigated. The chemical compositions of these extracts were analysed by GC-MS. Phenylethyl alcohol (78.38%) was found to be the main constituent of rose absolute, while citrenellol and geraniol were the major compounds (>55%) of rose essential oil and hydrosol. Tocopherol and carotene levels were determined by high performance liquid chromatography (HPLC) analysis. The levels of beta carotene (422.3±35.6 ppm), alpha tocopherol (2397.1±72.5 ppm) and gamma tocopherol (343.1±28.4 ppm) of rose absolute were found to be higher than that of essential oil and hydrosol. Their total phenolic contents were also evaluated. The total phenolic content of the tested extracts varied from 5.2 to 2134.3 GAE/mg L−1. Rose absolute and essential oil contained high levels of phenolics and demonstrated strong antibacterial activity against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 6538), Chromobacterium violaceum (ATCC 12472) and Erwinia carotovora (ATCC 39048) strains.

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  1. 1.

    Kaul VK, Singh V, Singh B (2000) Damask rose and marigold: prospective industrial crops. J Med Aromat Plant Sci 22:313–318

    CAS  Google Scholar 

  2. 2.

    Kürkçüoğlu M, Başer KHC (2003) Studies on Turkish rose concrete, “absolute and hydrosol”. Chem Nat Comp 39:457–464

    Article  Google Scholar 

  3. 3.

    Boskabady MH, Kiani S, Rakhshandah H (2006) Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol 106:377–382

    PubMed  Article  CAS  Google Scholar 

  4. 4.

    Rakhshandah H, Hosseini M (2006) Potentiation of pentobarbital hypnosis of by Rosa damascena in mice. Indian J Exp Biol 44:910–912

    PubMed  CAS  Google Scholar 

  5. 5.

    Kheirabadi M, Moghimi A, Rakhshande H, Rassouli MB (2008) Evaluation of the anticonvulsant activities of Rosa damascena on the PTZ induced seizures in wistar rats. JBS 8:426–430

    Article  Google Scholar 

  6. 6.

    Tabrizi H, Mortazavi SA, Kamalinejad M (1993) An in vitro evaluation of various Rosa damascena flower extracts as a natural antisolar agent. Int J Cosmet Sci 25:259–265

    Article  Google Scholar 

  7. 7.

    Kumar N, Bhandari P, Singh B, Bari SS (2008) Antioxidant activity and ultra-performance LC-electrospray ionization quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, R. bourboniana and R. brunonii. Food Chem Toxicol 47:361–367

    PubMed  Google Scholar 

  8. 8.

    Kovatcheva-Apostolova E, Milen G, Mladenka I, Leif S, Anja R, Mogens A (2008) Extracts of plant cell cultures of Lavandula vera and Rosa damascena as sources of phenolic antioxidants for use in foods. Eur Food Res Technol A 227:1243–1249

    Article  CAS  Google Scholar 

  9. 9.

    Arıdoğan BC, Baydar H, Kaya S, Demirci M, Özbaşar D, Mumcu E (2002) Antimicrobial activity and chemical composition of some essential oils. Arch Pharm Res 25:860–864

    PubMed  Article  Google Scholar 

  10. 10.

    Basim E, Basim H (2003) Antibacterial activity of Rosa damascena essential oil. Fitoterapia 74:394–396

    PubMed  CAS  Google Scholar 

  11. 11.

    Achuthan CR, Babu BH, Padikkala J (2003) Antioxidant and hepatoprotective effects of Rosa damascena. Pharm Biol 41:357–361

    Article  Google Scholar 

  12. 12.

    Özkan G, Sağdıç O, Göktürk-Baydar N, Baydar H (2004) Antioxidant and antibacterial activities of Rosa damascena flower extracts. Food Sci Technol Int 10:277–281

    Article  Google Scholar 

  13. 13.

    Gochev V, Wlcek K, Buchbauer G, Stoyanova A, Dobreva A, Schmidt E, Jirovetz L (2008) Comparative evaluation of antimicrobial activity and composition of rose oils from various geographic origins, in particular Bulgarian rose oil. Nat Prod Commun 3:1063–1068

    CAS  Google Scholar 

  14. 14.

    Hammer KA, Carson CF, Riley TV (1999) Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol 86:985–990 6

    PubMed  Article  CAS  Google Scholar 

  15. 15.

    Lampi AM, Kataja L, Kamal-Eldin A, Piironen V (1999) Antioxidant activities of α- and γ-tocopherols in the oxidation of rapeseed oil triacylglycerols. J Am Oil Chem Soc 76:749–755

    Article  CAS  Google Scholar 

  16. 16.

    Singleton VL, Rossi JR (1965) Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid. Am J Enology and Vitic 16:144–158

    CAS  Google Scholar 

  17. 17.

    Jirovetz L, Buchbauer G, Shahabi M (2002) Comparative investigations of essential oils and their SPME headspace volatiles of Rosa damascena from Bulgaria and Rosa centifolia from Morocco using GC-FID, GC–MS and olfactometry. J Essent Oil-Bear Plants 5:111–121

    CAS  Google Scholar 

  18. 18.

    Jirovetz L, Buchbauer G, Stoyanova A, Balinova A, Guangjiun Z, Xihan M (2005) Solid phase microextraction/gas chromatographic and olfactory analysis of the scent and fixative properties of the essential oil of Rosa damascena L. VF. Flavour Fragr J 20:7–12

    Article  CAS  Google Scholar 

  19. 19.

    Loghmani-Khouzani H, Sabzi-Fini O, Safari J (2007) Essential oil composition of Rosa damascena Mill cultivated in central Iran. Sci Iran 14:316–319

    CAS  Google Scholar 

  20. 20.

    Aydinli M, Tutas M (2003) Production of rose absolute from rose concrete. Flavour Fragr J 18:32–35

    Article  CAS  Google Scholar 

  21. 21.

    Cai YZ, Xing J, Sun M, Zhan ZQ, Corke H (2005) Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers. Agric Food Chem 53:9940–9948

    Article  CAS  Google Scholar 

  22. 22.

    Jassbi AR, Zamanizadehnajarib S, Tahara S (2003) Polyphenolic antioxidant constituents of Rosa persica. J Chem Soc Pak 25:323–327

    CAS  Google Scholar 

  23. 23.

    Lindsay DG, Astley SB (2002) European research on the functional effects of dietary antioxidants. EUROFEDA. Mol Aspects Med 23:1–38

    PubMed  Article  CAS  Google Scholar 

  24. 24.

    Tavani A, La Vecchia C (1999) Beta-carotene and risk of coronary heart disease. A review of observational and intervention studies. Biomed Pharmacother 53:409–416

    PubMed  Article  CAS  Google Scholar 

  25. 25.

    Etschmann MMW, Bluemke W, Sell D, Schrader J (2002) Biotechnological production of 2-phenylethanol. Appl Microbiol Biotechnol 59:1–8

    PubMed  Article  CAS  Google Scholar 

  26. 26.

    Fabre CE, Blanc PJ, Goma G (1998) 2-Phenylethyl alcohol: an aroma profile. Perfume Flavor 23:43–45

    CAS  Google Scholar 

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We thank to the Sebat Ltd., Isparta for kindly providing rose essential oil, hydrosol and rose absolute.

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Correspondence to Seyhan Ulusoy.

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Ulusoy, S., Boşgelmez-Tınaz, G. & Seçilmiş-Canbay, H. Tocopherol, Carotene, Phenolic Contents and Antibacterial Properties of Rose Essential Oil, Hydrosol and Absolute. Curr Microbiol 59, 554 (2009).

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  • Tocopherol
  • High Performance Liquid Chromatography
  • Total Phenolic Content
  • Carotene
  • Geraniol