Skip to main content
SpringerLink
Log in

Diversities Within Genotypes, Bioactivity and Biosynthetic Genes of Endophytic Actinomycetes Isolated from Three Pharmaceutical Plants

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

One hundred and fifty endophytic actinomycetes were isolated from three pharmaceutical plants, Annonaceae squamosal, Camptotheca acuminate and Taxus chinensis. Bioactivity test showed that 72.4% of the endophytic actinomycetes displayed inhibition against more than one indicator microorganism. In total, 9.3 and 10.7% showed the cytotoxicity and antioxidant activity, respectively. 3-Amino-5-hydroxybenzoic acid synthase (AHBA), ketosynthase (KS), cytochrome P450 hydroxylases (CYPs) and epoxidase (ES) encoding genes were found in 8.8, 23.8, 2.8 and 11.7% isolates, respectively, by genes screening. The identification based on traditional and molecular methods indicated that diverse genotypes of Streptomyces were distributed in the three pharmaceutical plants, and a few strains of Amycolatopsis were also found in the root of T. chinensis. These results indicated that endophytic actinomycetes associated with pharmaceutical plants could be a promising source of drug leads.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Augustine SK, Havsar SP, Kapadnis BPK (2005) A non-polyene antifungal antibiotic from Streptomyces albidoflavus PU23. J Biosci 30:201–211

    Article  PubMed  CAS  Google Scholar 

  2. Ayuso-Sacido A, Genilloud O (2004) New PCR primers for the screening of NRPS and PKS-I system in actinomycetes: detection and distribution of these biosynthetic gene sequences in major taxonomic groups. Microbial Ecol 49:10–24

    Article  CAS  Google Scholar 

  3. Blair S, Correa A, Madrigal B et al (1991) Plantas antimaláricas: una revisión bibliográfica. Universidad de Antioquia Medellín, p 214

  4. Cao LX, Qiu ZQ, You JL et al (2005) Isolation and characterization of endophytic streptomycete antagonists of fusarium wilt pathogen from surface-sterilized banana roots. FEMS Microbiol Lett 247:147–152

    Article  PubMed  CAS  Google Scholar 

  5. Castillo UF, Strobel GA, Ford EJ et al (2002) Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans. J Microbiol 148:2675–2685

    CAS  Google Scholar 

  6. Coenye T, Vandamme P (2003) Intragenomic heterogeneity between multiple 16S ribosomal RNA operons in sequenced bacterial genomes. FEMS Microbiol Lett 228:45–49

    Article  PubMed  CAS  Google Scholar 

  7. Courtois S, Cappellano CM, Ball M et al (2003) Recombinant environmental libraries provide access to microbial diversity for drug discovery from natural products. Appl Environ Microbiol 69:49–55

    Article  PubMed  CAS  Google Scholar 

  8. Fields AL, Runowicz CD (2003) Current therapies in ovarian cancer. Cancer Invest 21:148–156

    Article  PubMed  Google Scholar 

  9. Gauthier MJ, Shewan JM, Gibson DM et al (1975) Taxonomic position and seasonal variations in marine neritic environment of some gram-negative antibiotic-producing bacteria. J Gen Microbiol 87:211–218

    PubMed  CAS  Google Scholar 

  10. Hasegawa T, Takizawa M, Tanida S (1983) A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29:319–322

    Article  CAS  Google Scholar 

  11. Hwang YB, Lee MY, Park HJ et al (2007) Isolation of putative polyene-producing actinomycetes strains via PCR-based genome screening for polyene-specific hydroxylase genes. Process Biochem 42:102–107

    Article  CAS  Google Scholar 

  12. Lam KS (2006) Discovery of novel metabolites from marine actinomycetes. Curr Opin Microbiol 9:245–251

    Article  PubMed  CAS  Google Scholar 

  13. Lee SO, Gyung JC, Yong HC et al (2008) Isolation and characterization of endophytic actinomycetes from Chinese cabbage roots as antagonists to Plasmodiophora brassicae. J Microbiol Biotechnol 18(11):1741–1746

    PubMed  CAS  Google Scholar 

  14. Li J, Zhao GZ, Chen HH et al (2009) Antitumour and antimicrobial activities of endophytic streptomycetes from pharmaceutical plants in rainforest. Lett Appl Microbiol 47:574–580

    Article  Google Scholar 

  15. Lin X, Lu CH, Huang YJ et al (2007) Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity. World J Microbiol Biotechnol 23:1037–1040

    Article  Google Scholar 

  16. Liu ZH, Jiang CL (2004) The current biology and biotechnology of actinomycete. Science Publishing Company, Beijing

    Google Scholar 

  17. Miles D, Minckwitz GV, Seidman AD (2002) Combination versus sequential single-agent therapy in metastatic breast cancer. Oncologist 7:13–19

    PubMed  CAS  Google Scholar 

  18. Mosmann F (1983) Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65:55–63

    Article  PubMed  CAS  Google Scholar 

  19. Osorio E, Arango GJ, Jiménez N et al (2007) Antiprotozoal and cytotoxic activities in vitro of Colombian Annonaceae. J Ethnopharmacology 111:630–635

    Article  Google Scholar 

  20. Parejo I, Codina C, Petrakis C et al (2000) Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH free radical assay. J Pharmacol 44:507–512

    CAS  Google Scholar 

  21. Saikkonen K, Wali P, Helander M et al (2004) Evolution of endophyte-plant symbiosis. Trends Plant Sci 9:275–280

    Article  PubMed  CAS  Google Scholar 

  22. Schutz B, Boyle C, Draeger S et al (2001) Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 106:996–1004

    Google Scholar 

  23. Sessitsch A, Reiter B, Ulrike P (2002) Cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and actinomycetes-specific PCR of 16S rRNA genes. FEMS Microbiol Ecol 39:23–32

    Article  PubMed  CAS  Google Scholar 

  24. Shiring EB, Gotlieb D (1966) Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340

    Article  Google Scholar 

  25. Stamford TL, Stamford NP, Coelho LC et al (2002) Production and characterization of a thermostable glucoamylase from Streptosporangium sp. endophyte of maize leaves. Bioresour Technol 83:105–109

    Article  PubMed  CAS  Google Scholar 

  26. Stierle A, Strobel G, Stierle D (1993) Taxol and taxol production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260:214–216

    Article  PubMed  CAS  Google Scholar 

  27. Strobel G, Yang XS, Sears J et al (1996) Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology 142:435–440

    Article  PubMed  CAS  Google Scholar 

  28. Strobel G (2003) Endophytes as sources of bioactive product s microbes and infection. Microbes Infect 5:535–544

    Article  PubMed  CAS  Google Scholar 

  29. Wall ME, Wani MC (1996) Camptothecin and taxol: from discovery to clinic. J Ethnopharmacol 51:239–254

    Article  PubMed  CAS  Google Scholar 

  30. Wang JF, Li GL, Lu HY et al (2000) Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS Microbiol Lett 193:153–249

    Article  Google Scholar 

  31. Wang P (1986) A rapid method to detect the amino acid and sugar in actinomycete: TLC. Microbiology 13:228–231

    CAS  Google Scholar 

  32. Weniger B, Robledo S, Arango GJ et al (2001) Antiprotozoal activities of Colombian plants. J Ethnopharmacol 78:193–200

    Article  PubMed  CAS  Google Scholar 

  33. Williams ST, Goodfellow M, Alderson G (1989) Bergey’s manual of systematic bacteriology, vol 4. Williams & Wilkins, Baltimore, pp 2452–2492

    Google Scholar 

  34. Zhu N, Zhao PJ, Shen YM (2009) Selective isolation and ansamycin-targeted screenings of commensal actinomycetes from the ‘‘maytansinoids-producing’’ arboreal Trewia nudiflora. Curr Microbiol 58:87–94

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported through National Natural Sciences Foundation of China (grant no. 30500632). All of the authors are grateful to Prof. Bai Linquan from Shanghai Jiaotong University for helpful suggestions.

Author information

Authors and Affiliations

  1. The Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, P.O. Box 958, Xiamen, 361005, People’s Republic of China

    Yingying Wu, Chunhua Lu, Xiaoming Qian, Yaojian Huang & Yuemao Shen

Authors
  1. Yingying Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chunhua Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoming Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yaojian Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuemao Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yaojian Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, Y., Lu, C., Qian, X. et al. Diversities Within Genotypes, Bioactivity and Biosynthetic Genes of Endophytic Actinomycetes Isolated from Three Pharmaceutical Plants. Curr Microbiol 59, 475–482 (2009). https://doi.org/10.1007/s00284-009-9463-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-009-9463-2

Keywords

Search

Navigation