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Effect of Batch and Fed-Batch Growth Modes on Biofilm Formation by Listeria monocytogenes at Different Temperatures

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Abstract

The influence of Listeria monocytogenes (L. monocytogenes) biofilm formation feeding conditions (batch and fed-batch) at different temperatures on biofilm biomass and activity was determined. Biofilm biomass and cellular metabolic activity were assessed by Crystal Violet (CV) staining and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt (XTT) colorimetric method, respectively. Live/Dead staining was also performed in order to get microscopic visualization of the different biofilms. Results revealed that at refrigeration temperature (4°C) a higher amount of biofilm was produced when batch conditions were applied, while at higher temperatures the fed-batch feeding condition was the most effective on biofilm formation. Moreover, independently of the temperature used, biofilms formed under fed-batch conditions were metabolically more active than those formed in batch mode. In conclusion, this work shows that different growth modes significantly influence L. monocytogenes biofilm formation on abiotic surfaces as well as the metabolic activity of cells within biofilms.

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Acknowledgments

Authors gratefully acknowledge the helpful contribution of Lúcia Simões on the microscopy assays. This work was supported by Portuguese Science Foundation (FCT) through the project PPCDT/AGR/59358/2004. Diana Rodrigues and Pilar Teixeira acknowledge the financial support of FCT through Grants SFRH/BD/28887/2006 and SFRH/BPD/26803/2006, respectively.

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Authors and Affiliations

  1. IBB – Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Diana A. Rodrigues, Marta A. Almeida, Pilar A. Teixeira, Rosário T. Oliveira & Joana C. Azeredo

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  1. Diana A. Rodrigues
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  2. Marta A. Almeida
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  3. Pilar A. Teixeira
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  4. Rosário T. Oliveira
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  5. Joana C. Azeredo
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Correspondence to Joana C. Azeredo.

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Rodrigues, D.A., Almeida, M.A., Teixeira, P.A. et al. Effect of Batch and Fed-Batch Growth Modes on Biofilm Formation by Listeria monocytogenes at Different Temperatures. Curr Microbiol 59, 457–462 (2009). https://doi.org/10.1007/s00284-009-9460-5

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  • DOI: https://doi.org/10.1007/s00284-009-9460-5

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