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Virulence Increasing of Salmonella typhimurium in Balb/c Mice After Heat-Stress Induction of Phage Shock Protein A

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Abstract

Salmonella typhimurium is a potentially intracellular pathogen and is responsible for thousands of reported cases of acute gastroenteritis and diarrhea each year. Although many successful physiological and genetic approaches have been taken to conclude the key virulence determinants encoded by this organism, the total number of uncharacterized reading frames observed within the S. typhimurium genome suggests that many virulence factors remain to be discovered. This study was conducted to evaluate the role of heat induced phage shock protein A (PspA), in the pathogenicity of S. typhimurium. The stress proteins detected on sodium dodecyl sulfate-polyacrylamide gel electrophoresis were identified specifically by immunoblotting with polyclonal antibody against PspA. PspA was produced in response to heat stress at 45°C and it was over-expressed at 65°C. At this temperature, the stressed bacterial cells producing PspA were more virulent (16 folds greater) to female 6–8 week-old Balb/c mice. Correspondency between decrease in LD50 and increase in PspA production during heat stress and lower pathogenicity in non-producing cells that emerged during stress at 55°C represents PspA as an important virulence factor in heat stressed S. typhimurium.

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Acknowledgments

This work was supported by Young Researchers Club (YRC) in Tehran Sciences and Research Branch of IAU. We thank Ahmadreza Shoa Hassani for the helpful comments and benefit information.

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Authors and Affiliations

  1. Department of Microbiology, Fars Science and Research Branch of IAU, Shiraz, Fars, Iran

    Alireza Shoae Hassani

  2. Young Researchers Club (YRC) of Science and Research Branch of Islamic Azad University, Tehran, Iran

    Alireza Shoae Hassani & Amir Ghaemi

  3. Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran

    Nour Amirmozafari

  4. Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran

    Amir Ghaemi

Authors
  1. Alireza Shoae Hassani
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  2. Nour Amirmozafari
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  3. Amir Ghaemi
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Correspondence to Amir Ghaemi.

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Hassani, A.S., Amirmozafari, N. & Ghaemi, A. Virulence Increasing of Salmonella typhimurium in Balb/c Mice After Heat-Stress Induction of Phage Shock Protein A. Curr Microbiol 59, 446–450 (2009). https://doi.org/10.1007/s00284-009-9458-z

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  • DOI: https://doi.org/10.1007/s00284-009-9458-z

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